Deoxyribonuclease I (Dnase I)
Ibisobanuro
DNase I (Deoxyribonuclease I) ni endodeoxyribonuclease ishobora gusya ADN imwe cyangwa ebyiri.Iremera kandi ikomatanya imiyoboro ya fosifori kugirango itange monodeoxynucleotide cyangwa oligodeoxynucleotide imwe cyangwa ebyiri-ebyiri hamwe na fosifate mumatsinda ya 5'-terminal na hydroxyl kuri 3'-terminal.Igikorwa cya DNase I giterwa na Ca 2+ kandi gishobora gukoreshwa na ion zingana nicyuma nka Mn 2+ na Zn 2+.5 mM Ca 2+ irinda enzyme hydrolysis.Imbere ya Mg 2+, enzyme irashobora kumenya kubushake no gutobora urubuga urwo arirwo rwose rwa ADN.Imbere ya Mn 2+, imirongo ibiri ya ADN irashobora kumenyekana icyarimwe kandi igafatirwa ahantu hamwe kugirango ibe ibice bya ADN bigororotse cyangwa ibice bya ADN bifatanye hamwe na nucleotide 1-2.
Imiterere yimiti
Igisobanuro
Igice kimwe gisobanurwa nkubunini bwa enzyme izatesha agaciro rwose 1 µg ya ADN ya pBR322 muminota 10 kuri 37 ° C.
Ibisobanuro
| Ibizamini | Ibisobanuro |
| Isuku (SDS-PAGE) | ≥ 95% |
| Igikorwa cya Rnase | Nta Gutesha agaciro |
| gDNA Kwanduza | Copy 1 kopi / μL |
Gutwara no kubika
Ubwikorezi:Yoherejwe munsi ya 0 ° C.
Ububiko:Ubike kuri -25 ~ -15 ° C.
Basabwe kongera kugerageza Ubuzima:Imyaka 2
