Recombinant Trypsin
Ibyiza
1.Ntinkomoko yinyamaswa
2.Isuku ryinshi
3.Gucunga neza inzira
4.Ubuziranenge buhamye
Ibisobanuro
Trypsin ikuraho C-terminal peptide ihuza lysine na arginine, ishobora gutesha agaciro poroteyine zihuza ingirabuzimafatizo.Urutonde rwa aminide acide ya recombinant trypsin yakozwe na Hzymes Biotech ni imwe nki ya pcine pancreas ikomoka kuri trypsin, kandi ikorwa na recombinant Escherichia coli imvugo.Irashobora gusimbuza gakondo ya trypsin kugirango igogorwa rya selile mu nkingo, ingirangingo fatizo, kuvura ingirabuzimafatizo, gusuzuma ibiyobyabwenge, antibodies nizindi nzego.Aprotinin, soya ya trypsin inhibitor, nibindi birashobora kubuza gutandukanya ibikorwa bya trypsin.
Recombinant trypsin ifite imitungo imwe na trypsin isanzwe ikuramo.Ibicuruzwa byakorewe mumahugurwa ya GMP.Nta nkomoko y’ibikomoka ku nyamaswa ikoreshwa muri fermentation, kweza nibindi bintu byanyuma.Muri icyo gihe, umusaruro no kugenzura ubuziranenge byemewe, bivamo urwego rwo hejuru rwo guhuza hagati yitsinda.
Imiterere yimiti
Igisobanuro
25 ℃, pH 7.6, 3.2ml igisubizo cyibisubizo (1cm inzira yumucyo), igice kimwe cya trypsin (U) cyasobanuwe nk an
kwiyongera kwa 0.003 mugiciro cyo kwinjiza kuri 253nm na hydrolysis enzymatique ya BAEE kumunota.
Imikoreshereze y'ibicuruzwa
Umuco w'akagari
• Tissue block igogora, kugura selile yibanze.
• Gusya ibice bya selile zifatika.
• Umuco w'akagari ukoresheje microcarrier.
• Gusya witonze ingirabuzimafatizo.
• Ubudahangarwa bw'umubiri, n'ibindi.
Kuri poroteyine ya recombinant
• Umusemburo wa insuline.
• Urutonde rwa poroteyine, ikarita ya peptide.
• Inzira yihariye ya proteolyique nkubushakashatsi bwa proteomics
Ibisobanuro
| Ibizamini | Ibisobanuro |
| Kugaragara | Ifu yera cyangwa hanze |
| Gukemura (10mg / ml) | Sobanura igisubizo kitagira ibara |
| Isuku | Try-trypsin ≥ 70% ， α-trypsin ≤ 20% |
| Igikorwa cyihariye (Ifu) | ≥2500U / mg |
| E.Coli yakira ibisigazwa bya poroteyine | ≤0.01% |
| Endotoxine (LAL-Ikizamini) | EU20 EU / mg |
Gutwara no kubika
Ubwikorezi:Yoherejwe munsi ya 2-8 ° C.
Ububiko:Ubike kuri -2-8 ° C, nyuma yo gushonga hamwe na 1mM hcl cyangwa 50nM HAC, bigomba kubikwa munsi ya -25 ~ -15 ° C
Basabwe kongera kugerageza Ubuzima:Imyaka 2
