Ultra Nuclease Assay Kit (ELISA)
Ibisobanuro
Iyi Ultra Nuclease ELISA kit ni umusenyi wich ELISA ugomba gukorwa muburyo bwa microplate.Icyitegererezo gishobora kuba kirimo endonuclease yashyizwe mumariba ya microtiter yamashanyarazi yabanje gushyirwaho hamwe na affinity yasukuye anti-endonuclease gufata antibody.Nyuma ya incububasi nintambwe yo gukaraba ikuramo ibice bidafunze, hiyongeraho enzyme-conjugated, anti-endonuclease detector antibody.Imyitwarire yubudahangarwa itera gukora sandwich igizwe na antibody ikomeye-endonuclease-enzyme yanditseho antibody.Nyuma yintambwe yanyuma yo gukaraba, igisubizo cya substrate cyongewe kumariba kandi kigakorwa, bikavamo iterambere ryamabara.Ubucucike bwa optique bupimwa mu buryo bwa fotometrike kandi buringaniye na
analyte kwibanda kuboneka mumariba.Kwibanda kwa endonuc Please mubyitegererezo bitazwi birashobora kubarwa hashingiwe kumurongo uhuye.
Imiterere yimiti
Igisobanuro
Ingano ya enzyme ikoreshwa muguhindura agaciro ka △ A260 kuri 1.0 muri 30min
kuri 37 ° C, pH 8.0, bihwanye na 37μg intanga ngabo ya salmon ADN ikata muri oligonucleotide, yasobanuwe nkigice gikora.
Imikoreshereze n'imikoreshereze
• Kuraho acide nucleic acide yibicuruzwa byinkingo, kugabanya ibyago byuburozi bwa nucleic aside isigaye kandi bitezimbere umutekano wibicuruzwa.
• Kugabanya ubwiza bwamazi yibiryo biterwa na acide nucleic, kugabanya igihe cyo gutunganya no kongera umusaruro wa proteine.
• Kuraho aside nucleic yapfunyitse agace (virusi, umubiri ushiramo, nibindi), bifasha kurekura no kweza ibice.
• Kuvura nuclease birashobora kunoza gukemura no kugarura icyitegererezo cya chromatografiya, electrophoreis hamwe nisesengura rya blotting.
• Mu kuvura gene, aside nucleic ikurwaho kugirango ibone virusi zifitanye isano na adeno.
Ibisobanuro
| Ibizamini | Ibisobanuro |
| Umupaka muto wo gutahura | 0,6 ng / mL |
| Umubare ntarengwa wo kubara | 0.2 ng / mL |
| Icyitonderwa | Imbere muri CV≤10% |
Gutwara no kubika
Ubwikorezi:Yoherejwe munsi ya 0 ° C.
Ububiko:Ubike kuri -2-8 ° C, ufunguye reagent ihamye ibyumweru 6
Basabwe kongera kugerageza Ubuzima:Umwaka 1
