T7 RNA Poymerase (Kwibanda cyane)
Ibisobanuro
T7 RNA Polymerase ni ADN iterwa na RNA-olymerase kuva T7 fage possess ifite ibikorwa bikomeye kandi byihariye 5 ´ → 3 ´ RNA polymerase.T7 RNA Poplymerase ifite umwihariko mwinshi kuri T7 ikurikirana kandi izahuza byinshi bya RNA bivuye mubice bya ADN byinjijwe hepfo ya porotokoro.
Imiterere yimiti
Igisobanuro
Igice kimwe gisobanurwa nkubunini bwa enzyme isabwa kugirango yinjize 1 nmol ATP mubikoresho bya aside-idashonga mumasaha 1 kuri 37 ° C.
Ibisobanuro
| Ibizamini | Ibisobanuro |
| Intungamubiri za poroteyine | ≥95% |
| Nickase | Nta n'umwe wamenyekanye |
| Rnase | Nta n'umwe wamenyekanye |
| Endonuc Please | Nta n'umwe wamenyekanye |
| Exonuc Please | Nta n'umwe wamenyekanye |
| Umushoramari Umwihariko | Ntibishobora kugaragara kubicuruzwa bidafite akamaro |
| E.coli gDNA | ≤0.1 pg / 50U |
| Endotoxin | LAL-Ikizamini, ≤10EU / mg |
Gutwara no kubika
Ubwikorezi:munsi ya 0 ° C.
Ububiko:Ibigize byose bigomba kubikwa kuri -25 ～ -15 ° C.
Basabwe kongera kugerageza Ubuzima:Imyaka 2
ibicuruzwa bifitanye isano
Andika ubutumwa bwawe hano hanyuma utwohereze
