BspQI Kubuza Endonuc Please
Ibisobanuro
BspQI irashobora kugaragazwa muri E. coli ishobora kumenya imbuga zihariye kandi ikorerwa mubipimo bya GMP.Ikoreshwa cyane mugushushanya gene, cloni nizindi nzego.Iyi misemburo igabanya byihuse ADN kugirango ikoreshwe neza.
5 '······ GGTCTC (N) 1 ······ 3'
3 '······ CCAGAG (N) 4 ······· 5'
Imiterere yimiti
Igisobanuro
Igice kimwe gisobanurwa nkubunini bwa enzyme isabwa kugirango igogwe 1 µg ya λ ADN mu isaha 1 kuri 50 ° C mubunini bwa reaction ya 50 µl.
Ibiranga ibicuruzwa
• Kubuza endonuclease ifite ibikorwa byimisemburo myinshi kandi irangiza igogorwa vuba.
• Igikorwa cyo hasi kidasanzwe cyihariye kugirango tumenye neza "scalpel".
• Nta sisitemu ya BSA, igabanya umwanda w’ubushyuhe.
Ibisobanuro
| Ibizamini | Ibisobanuro |
| Intungamubiri za poroteyine | ＞ 98% |
| Protease ibisigazwa | Nta n'umwe wamenyekanye |
| Kwihuza no kugabanya gutahura | Gukora neza neza ＞ 95% |
| DNase | Nta n'umwe wamenyekanye |
| Nikaknase | Nta n'umwe wamenyekanye |
Gutwara no kubika
Ubwikorezi:Yoherejwe munsi ya 0 ° C.
Ububiko:Ubike kuri -20 ° C.
Basabwe kongera kugerageza Ubuzima:2year
ibicuruzwa bifitanye isano
Andika ubutumwa bwawe hano hanyuma utwohereze
