Fosifata ya alkaline (ALP)

Alkaline Phosphatase ikomoka kumurongo wa recombinant E. coli itwara gene TAB5.Enzyme itera dephosifora ya 5´ na 3´ ya ADN na fosifomone ya RNA.Nanone, hydrolyses ribose, kimwe na deoxyribonucleoside triphosphates (NTPs na dNTPs).TAB5 Fosifata ya alkaline ikora kuri 5´ isohoka, 5´ isubirwamo kandi itagaragara.Fosifata irashobora gukoreshwa mubikorwa byinshi bya biyolojiya ya biologiya, nka clon cyangwa probe end labels kugirango ikureho fosifora ya ADN cyangwa RNA.Mu bushakashatsi bwakoronijwe, dephosifora irinda ADN umurongo wa plasmid umurongo wo kwikuramo.Irashobora kandi gutesha agaciro dNTPs zidafite uruhare muri PCR kugirango zitegure icyitegererezo cya ADN ikurikirana.Enzyme irakorwa rwose kandi kuburyo budasubirwaho no gushyushya 70 ° C muminota 5, bityo bigatuma ikuramo fosifata mbere yo kuburana cyangwa kurangiza kuranga bitari ngombwa.