BsaI Kubuza Endonuc Please
Ibisobanuro
Enzyme yo kubuza BsaI ni ubwoko bwa II bwo kubuza endonuclease ikomoka kuri proteine ya recombinant yashizweho na gene ya BspQI muri Bacillus sphaericus yagaragajwe na E.coli.Kubuza endonuclease ikoreshwa cyane mugushushanya gene, gukoroniza nizindi nzego.Iyi misemburo igabanya ADN byihuse kugirango igaragaze neza umurongo wa Bsa I, IIs ibuza endonuclease kubuza endonuclease, ikomoka kumurongo wa recombinant E. coli itwara gene ya BsaI yahinduwe kandi ihinduwe na Bacillus stearothermophilus.zifite umwihariko umwe na enzymes kavukire no kugabanya ibikorwa byinyenyeri.Urubuga rwo kumenyekanisha hamwe na site ya clavage nuburyo bukurikira:
5 '······ GGTCTC (N) ··········'
3 '······ CCAGAG (NNNNN) ······ 5'
Imiterere yimiti
Ibiranga ibicuruzwa
• Kubuza endonuclease ifite ibikorwa byimisemburo myinshi kandi irangiza igogorwa ryihuse.
• Igikorwa cyo hasi kidasanzwe cyihariye kugirango tumenye neza "scalpel".
• Nta sisitemu ya BSA, igabanya umwanda w’ubushyuhe.
Ibisobanuro
| Ibizamini | Ibisobanuro |
| Igikorwa cya Enzyme | 20 U / µL |
| Isuku (SDS-PAGE) | ≥95% |
| Rnase | Nta n'umwe wamenyekanye |
| Igikorwa kidasanzwe cya DNase | Kutamenyekana |
| Igikorwa c'inyenyeri | Kutamenyekana |
| Kuburana no Gusubiramo | Nyuma yo gusya, ibice bya ADN birashobora guhuzwa.Kandi ibi bice byahujwe birashobora gusubirwamo na Bsal. |
| Endotoxine (LAL-ikizamini) | EU10 EU / mg |
| ADN ya ADN | Gukoporora / 2U |
Kugenzura ubuziranenge
Isuku ≥ 95%, Nta DNase, Igikorwa cya RNase, Ibisigisigi bya ADN byakiriye ≤ 100pg / mg, ibisigisigi bya poroteyine yakira ≤ -50ppm, ibisigisigi bya endotoxine ≤ 10EU / mg, Nta gikorwa cya protease, sterile, kitarimo mycoplasma
Gusaba
Gucukura plasmid kugirango utegure igice cya ADN umurongo ugizwe na Poly (A / T / C / G);
Gutondekanya plasmid icyitegererezo mbere muri vitro transcript;
Kubuza gusya;
Igisobanuro
Igice kimwe gisobanurwa nkubunini bwa enzyme isabwa kugirango igogwe 1 µg ya ADN igenzura imbere mumasaha 1 kuri 37 ° C mubisubizo bya 50 µL
Gutwara no kubika
Ubwikorezi:Yoherejwe munsi ya -15 ° C.
Ububiko:Ubike kuri -25 ~ -15 ° C.
Basabwe kongera kugerageza Ubuzima:Umwaka 1
