Bst ADN Polymerase
Ibisobanuro
Bst ADN Polymerase V2 ni homologiya ya Bacillus Stearothermophilus ADN polymase ya 1 (Bst ADN polymerase V1) kandi yateguwe no kwigana mudasobwa.Bst ADN polymerase V2 ifite 5´ → 3´ ibikorwa bya polymerase nigikorwa gikomeye cyo gusimbuza urunigi, ariko ntigifite ibikorwa bya 5´ → 3´.Ugereranije nubwoko bwa Bst ADN polymerase, enzyme irashobora kongera neza umuvuduko wo kongera imbaraga, umusaruro, kwihanganira umunyu hamwe nubushyuhe bwumuriro, nibindi.
Buffer y'Ububiko:10 mM Tris-HCl pH 7.1，50 mM KCl ， 0.1 mM EDTA ， 1 mM DTT ， 0.1% Triton X-100，50% Glycerine
Imiterere yimiti
Ibigize
| Ibigize | 400U | 800U | 40000u |
| ADN ADN Polymerase | 0.1 mL | 1 mL | 1 0mL |
| Bst Buffer | 1.5 mL | 2 × 1.5 mL | 3 × 10 mL |
| MgSO4 (100 mM) | 1.5 mL | 2 × 1.5 mL | 2 × 10 mL |
Ibisobanuro
| Ibintu byo kwipimisha | Ibisobanuro | Ibisubizo |
| Intungamubiri za poroteyine | ≥95% | Pass |
| Endonuc Please Igikorwa | Ntibimenyekana | Pass |
| Exonuclease Igikorwa | Ntibimenyekana | Pass |
| Igikorwa cya Rnase | Ntibimenyekana | Pass |
| Ntabwo ari umwihariko | Ntibimenyekana | Pass |
Porogaramu
Kwiyongera kwa Isothermal.
Urutonde rwa ADN hamwe nibintu byinshi bya GC
Urukurikirane rwihuse ruva kuri nanogramu ingano ya ADN yerekana.
Kohereza no kubika
Ubwikorezi:Amapaki
Uburyo bwo kubika:Ububiko kuri -25 ～ -15 ℃.
Basabwe kongera kwipimisha:Amezi 18
Icyitonderwa
Irinde gukonjesha inshuro nyinshi.Igicuruzwa gifite agaciro mumezi 18.
