RT-LAMP Reagent (-20 ℃)
Ibisobanuro
LAMP kuri ubu ikoreshwa cyane muburyo bwa tekinoroji ya isothermal amplification.Ikoresha primers 4-6 ishobora kumenya uturere 6 twihariye kuri gen igenewe, kandi ishingiye kubikorwa bikomeye byo kwimura imirongo ya Bst ADN polymerase.Hariho uburyo bwinshi bwo gutahura LAMP, harimo uburyo bwo gusiga irangi, uburyo bwa pH colimetric, uburyo bwo guhungabana, HNB, calcein, nibindi. RT-LAMP ni ubwoko bumwe bwa LAMP reaction hamwe na RNA nkicyitegererezo.RT-LAMP Kit (UDG plus) yakozwe hifashishijwe uburyo bwa fluorescence kandi ikubiyemo Bst DNA Polymerase, thermotolerant reverse transcriptase, murine RNase inhibitor, na UDGase.Iki gikoresho cya LAMP gifite sensibilité yo hejuru, primer nziza ikoreshwa, hamwe nibikorwa byiza muburyo bwihariye, kwihanganira, no gutuza.
Ibigize
| 100T | 1000T |
| 600μL | 6mL |
| 100 μL | 1 mL |
1) Buffer ya Bst irimo dN (U) TP na SYTO-9, nibindi, na 1 × Bst Buffer isanzwe irimo 8 mmol / L Mg2 +;
2) Bst Enzyme ivanze (UDG wongeyeho) irimo UDGase, Transcriptase, Bst Ⅱ Polymerase, MRI;
Porogaramu
Amatara
Kohereza no kubika
Ubwikorezi:Amapaki
Uburyo bwo kubika:Ubike kuri -20 ℃
Ubuzima bwa Shief:Amezi 6
Icyitonderwa
Kubwumutekano wawe nubuzima bwawe, nyamuneka wambare amakoti ya laboratoire hamwe na gants imwe ikoreshwa.
