Ultra Nuclease
Ibisobanuro
UltraNuclease ni endonuclaese yakozwe na genetique ikomoka kuri Serratia marcescens, ishoboye gutesha agaciro ADN cyangwa RNA, haba inshuro ebyiri cyangwa imwe ihagaze, umurongo cyangwa umuzenguruko muburyo butandukanye, yangiza rwose aside nucleic muri 5'-monophosphate oligonucleotide hamwe na 3-5 uburebure shingiro.
Nyuma yo guhindura ingengabihe ya geneti, ibicuruzwa byasembuwe, bigaragazwa, kandi bisukurwa muri Escherichia coli (E. coli), bigabanya ubukonje bwa selile ndengakamere na lysate ya selile mubushakashatsi bwa siyanse, ariko kandi binanoza imikorere yo kweza no gukora ubushakashatsi bwa poroteyine.Irashobora kandi gukoreshwa mubuvuzi bwa gene, kweza virusi, gutanga urukingo, proteyine na polysaccharide yimiti yimiti nkibisigisigi bya nucleic aside ikuraho reagent.
Imiterere yimiti
Igisobanuro
Ingano ya enzyme yakoreshejwe muguhindura agaciro ka △ A260 kuri 1.0 muri 30min kuri 37 ° C, pH 8.0, bihwanye na 37μg intanga ngabo ya salmon ADN ikata muri oligonucleotide, yasobanuwe nkigice gikora.
Imikoreshereze n'imikoreshereze
• Kuraho acide nucleic acide yibicuruzwa byinkingo, kugabanya ibyago byuburozi bwa nucleic aside isigaye kandi bitezimbere umutekano wibicuruzwa.
• Kugabanya ubwiza bwamazi yibiryo biterwa na acide nucleic, kugabanya igihe cyo gutunganya no kongera umusaruro wa proteine.
• Kuraho aside nucleic yapfunyitse agace (virusi, umubiri ushiramo, nibindi), bifasha kurekura no kweza ibice.
• Kuvura nuclease birashobora kunoza gukemura no kugarura icyitegererezo cya chromatografiya, electrophoreis hamwe nisesengura rya blotting.
• Mu kuvura gene, aside nucleic ikurwaho kugirango ibone virusi zifitanye isano na adeno.
Ibisobanuro
| Ibizamini | Ibisobanuro |
| Ibisobanuro | Birasobanutse kandi bitagira ibara |
| Igikorwa | U 250 U / ul |
| Igikorwa cyihariye | ≥1.1 * 106U / mg |
| Isuku （SDS-URUPAPURO） | ≥ 99.0% |
| Protease | Nta n'umwe wamenyekanye |
| Bioburden | ＜ 10 cfu / 100,000U |
| Endotoxine (LAL-ikizamini) | ＜ 0.25EU / 1.000U |
Gutwara no kubika
Ubwikorezi:Yoherejwe munsi ya 0 ° C.
Ububiko:Ubike kuri -25 ~ -15 ° C.
Basabwe kongera kugerageza Ubuzima:Umwaka 2 （irinde gukonjesha t
