Taqman Multiplex qPCR Imbere
Injangwe No: HCB5061B
Taqman Multiplex qPCR Premix master mix ni antibody nshya ya antibody yahinduwe Taq ADN Polymerase fluroescence ingano ya qPCR irashobora gukoreshwa muri reagent ya lyophilisation.Irakwiriye kuri FAM / HEX / TET / JOE / ROX n'ibindi.Reagent irimo hotstart Byihuta Taq ADN Polymerase, DNTP nibindi bikoresho bishobora gukama kubusa kumashini.Igicuruzwa ntabwo kirimo amarangi yakosowe na ROX kandi arakwiriye muburyo butandukanye bwa PCR.
Ibigize
Lyo-Yiteguye TaqMan qPCR Ivanga
Ububiko
Ibicuruzwa bigomba kubikwa kuri -25 ~ -15 ℃ kure yumucyo kumwaka 1.
Amabwiriza
1.Sisitemu yo kwitwara
| Ibigize | Umubumbe (μL) | Kwibanda kwanyuma |
| Enzyme Kuvanga | 20 | - |
| Primer Primer (10μM) | x | 0.1-0.5μM |
| Probe Primer (10μM) | x | 50-250nM |
| Icyitegererezo cya ADN | 1-25 | - |
| dd H.2O | kugeza kuri 25 | - |
Gahunda ya reaction
| Intambwe | Ubushuhe. | Igihe | Amagare |
| Gutandukana kwambere | 95 ℃ | 5min | 1 |
| Amplification reaction | 95 ℃ | 15sec | 45 |
| Kwiyongera / Kwagura | 60 ℃ | 30sec |
Inyandiko:Mubihe bidasanzwe ubushyuhe bwa annealing / kwaguka burashobora guhinduka ukurikije primer Tm agaciro, 60 ° C birasabwa.
Inzira ya Lyophilisation
| Inzira | Ubushuhe. | Shiraho igihe | Igihe | Umuvuduko |
| Mbere yo gukonjesha | -45 ℃ | 10min | 60min | - |
|
Kuma | -45 ℃ | 10min | 60min | 0.01mbar |
| -40 ℃ | 10min | 600min | 0.01mbar | |
| -35 ℃ | 10min | 120min | 0.01mbar | |
| -30 ℃ | 20min | 60min | 0.01mbar | |
| -20 ℃ | 20min | 60min | 0.01mbar | |
| -10 ℃ | 20min | 60min | 0.01mbar | |
| 0 ℃ | 20min | 60min | 0.01mbar | |
|
Kuma kabiri | 5 ℃ | 10min | 20min | 0.01mbar 0.01mbar |
| 10 ℃ | 10min | 20min | ||
| 15 ℃ | 10min | 20min | 0.01mbar | |
| 20 ℃ | 10min | 20min | 0.01mbar | |
| 25 ℃ | 10min | 240min | 0.01mbar |
Inyandiko
1. Iki gicuruzwa nikoreshwa mubushakashatsi gusa.
2. Nyamuneka kora ikoti ya laboratoire hamwe na gants imwe ikoreshwa, kubwumutekano wawe.
