Ribonuc Please A (RNase A)

Ribonuclease A (RNase A) ni polypeptide imwe imwe irimo imigozi 4 ya disulfide ifite uburemere bwa molekile hafi 13.7 kDa.RNase A ni endoribonuclease itesha agaciro cyane RNA imwe imwe kuri C na U.By'umwihariko, clavage imenya isano ya fosifori yakozwe na 5'-ribose ya nucleotide hamwe nitsinda rya fosifate kuri 3'-ribose ya nucleotide ya pyrimidine yegeranye, kugirango 2 ', 3' - Fosifike ya sikeli ihindurwe na hydrolyzed ihuye nayo. 3'-nucleoside fosifate (urugero, pG-pG-pC-pA-pG yashizwemo na RNase A kugirango itange pG-pG-pCp na A-PG).RNase A niyo ikora cyane mugukata RNA imwe imwe kandi ikora muburyo butandukanye bwo kwitwara: kumyunyu muke (0 kugeza 100 mM NaCl), irashobora gukoreshwa mugukata RNA imwe, RNA ikubye kabiri, na RNA imirongo ya RNA-ADN ivanze.mugihe umunyu mwinshi (≥0.3 M), RNase A irashobora gutandukanya RNA imwe.