DATP / dCTP / dGTP / dTTP / DUTP (100mM)
Ibisobanuro
Ibicuruzwa nibisubizo bitagira ibara.Irakwiriye kubushakashatsi bwibinyabuzima butandukanye busanzwe nka amplification ya PCR, igihe nyacyo PCR, cDNA cyangwa synthesis ya ADN isanzwe, uko ADN ikurikirana hamwe na label.Irashobora kuvangwa n'amazi meza cyane, hanyuma igahinduka kuri pH 7.0 hamwe numuti mwinshi wa NaOH, hamwe nubuziranenge ≥ 99% (HPLC).Nyuma yo gutahura, ntabwo irimo DNase, RNase na fosifotase.Irashobora gukoreshwa mu buryo butaziguye muburyo butandukanye busanzwe bwa biologiya nka PCR.
Imiterere yimiti
Kugaragaza neza
| Ibintu byo kwipimisha | Ibisobanuro |
| Kugaragara | Sobanura igisubizo kitagira ibara |
| Kwibanda | 100mM ± 3% |
| Isuku (HPLC) | ≥99% |
| PH(22-25 ° C) | 7.0 ± 0.1 |
| Kugenzura Ubuziranenge1.Iyi myiteguro nta DNase yanduye na RNase 2. Iyi ninshi ya DNTP yageragejwe hamwe na Taq ADN polymerase na Pfu ADN polymerase Iyi ninshi ya d NTP ifite imikorere yageragejwe hamwe na Q-PCR | |
Porogaramu
Intera ndende PCR (20 kb)
cDNA synthesis hamwe na RT-PCR
Igihe nyacyo PCR
PCR isanzwe
Ubudahemuka bukabije PCR
Kohereza no kubika
Ubwikorezi:Amapaki
Uburyo bwo kubika:Ubike kuri -25 ～ -15 ℃
Basabwe kongera kwipimisha:Imyaka 2
Inyandiko
1) Irashobora gushonga mubushyuhe bwicyumba.Nyuma yo guseswa, igomba kubikwa mu gasanduku ka barafu cyangwa koga.Nyuma yo gukoreshwa, igomba kubikwa kuri -25～-15 ℃ ako kanya.
2) Kubwumutekano wawe nubuzima bwawe, nyamuneka wambare amakoti ya laboratoire hamwe na gants imwe ikoreshwa kugirango ikore.
