DNTP ivanga (25mM buri / 10mM buri umwe)
Ibisobanuro
Iki gicuruzwa nigisubizo cyamazi kitagira ibara, DNTP ivanze nigisubizo kibanziriza dATP, dCTP, dGTP na dTTP, buri kimwe gifite ubunini bwa mmol 100 / L, gikwiranye nubushakashatsi butandukanye bwibinyabuzima bwa molekuline busanzwe nka amplification ya PCR, igihe nyacyo PCR, cDNA cyangwa ADN isanzwe, ADN ikurikirana hamwe na label.Irashobora kuvangwa n'amazi meza cyane, hanyuma igahinduka kuri pH 7.0 hamwe numuti mwinshi wa NaOH, hamwe nubuziranenge ≥ 99% (HPLC).Nyuma yo gutahura, ntabwo irimo DNase, RNase na fosifotase.Irashobora gukoreshwa mu buryo butaziguye muburyo butandukanye busanzwe bwa biologiya nka PCR.
Kugaragaza neza
| Ibintu byo kwipimisha | Ibisobanuro |
| Kugaragara | Sobanura igisubizo kitagira ibara |
| Kwibanda | 25mM buri / 10mM imwe |
| Isuku (HPLC) | ≥99% |
| PH(22-25 ° C) | 7.0 ± 0.5 |
| Kugenzura UbuziranengeIyi myiteguro nta DNase yanduye Ibi byinshi bya DNTP byageragejwe hamwe na Taq ADN polymerase na Pfu ADN polymerase Iyi ninshi ya d NTP ifite imikorere yageragejwe hamwe na Q-PCR | |
Porogaramu
Intera ndende PCR (20 kb)
cDNA synthesis hamwe na RT-PCR
Igihe nyacyo PCR
PCR isanzwe
Ubudahemuka bukabije PCR
Kohereza no kubika
Ubwikorezi:Amapaki
Uburyo bwo kubika:Ubike kuri -25 ～ -15 ℃
Basabwe kongera kwipimisha:Imyaka 2
