Homocysteine (HCY)
Ibisobanuro
Homocysteine (HCY) ikoreshwa mugutahura homocysteine mumaraso yabantu.Homocysteine (Hcy) ni sulfure irimo aside amine ikorwa na metabolism ya methionine.80% ya Hcy ihujwe na poroteyine binyuze mu maraso ya disulfide mu maraso, kandi igice gito gusa cya homocysteine yubusa kigira uruhare mukuzenguruka.Urwego rwa Hcy rufitanye isano rya hafi n'indwara z'umutima.ni ikintu cyingenzi gishobora gutera indwara z'umutima.Ubwiyongere bwa Hcy mu maraso butera urukuta rw'imitsi y'amaraso kwangiza imiyoboro y'amaraso, biganisha ku gutwika no gufata plaque ku rukuta rw'imitsi, amaherezo bigatuma habaho guhagarika amaraso mu mutima.Ku barwayi barwaye hyperhomocystinuria, inenge zikomeye zishingiye ku ngirabuzima fatizo zigira ingaruka kuri Hcy metabolism, bikaviramo hyperhomocysteinemia.Ubusembwa bworoheje bwa genetike cyangwa kubura imirire ya vitamine B bizajyana no kuzamuka kwa Hcy mu buryo butagereranywa cyangwa bworoheje, nabwo bizongera ibyago byo kurwara umutima.Hejuru ya Hcy irashobora kandi gutera ubumuga kuvuka nka neural tube inenge hamwe no kuvuka nabi.
Imiterere yimiti
Ihame ry'ikizamini
Oxidized Hcy ihindurwamo Hcy yubuntu, kandi Hcy yubuntu ikora hamwe na serine munsi ya catalizike ya CBS kugirango itange L-cystathionine.L-cystathionine itanga Hcy, pyruvate na NH3 munsi ya catalizike ya CBL.Pyruvate iterwa niyi reaction irashobora kugaragazwa na lactate dehydrogenase LDH na NADH, kandi igipimo cyo guhindura NADH kuri NAD gihuye neza nibiri muri Hcy murugero.
Gutwara no kubika
Ubwikorezi:2-8 ° C.
Igihe cyo kubika no kwemeza:Reagents idafunguye igomba kubikwa kuri 2-8 ° C mu mwijima, kandi igihe cyemewe ni amezi 12;nyuma yo gufungura, reagent igomba kubikwa mu mwijima kuri 2-8 ° C, kandi igihe cyemewe ni ukwezi 1 mugihe nta mwanda uhari;reagent ntigomba gukonjeshwa.
Icyitonderwa
Icyitegererezo cyibisabwa: Icyitegererezo ni serumu cyangwa plasma nshya (heparin anticoagulation, 0.1mg heparin irashobora kurwanya antikaguline 1.0ml).Nyamuneka centrifuge plasma ako kanya nyuma yo gukusanya amaraso, cyangwa gukonjesha na centrifuge mugihe cyisaha 1.
