Multiplex Intambwe imwe RT-qPCR Probe Kit (UDG Plus)
Ibisobanuro
Multiplex Intambwe imwe RT-qPCR Kit (UDG Plus) nigikoresho gishingiye kuri probe ishingiye kuri RT-qPCR igikoresho cyihuse gikwiranye na PCR imwe cyangwa plex nyinshi igereranya PCR ikoresheje RNA nk'icyitegererezo (nka virusi ya RNA).Byakoreshejwe mukwongerera ADN inshuro ebyiri kuva RNA inyandikorugero imwe kugirango yemererwe byihuse mugihe nyacyo cya RNA.Muntambwe yo kwandukura intambwe ihinduranya inyandiko ihuza molekile ya ADN imwe (cDNA) yuzuzanya nicyitegererezo cya RNA.
Inzira yo Kwitabira
Ibigize
| Ibigize | 200rxns | 1.000 | 10,000 rxns |
| Ubusa ddH2O | 3 * 1ml | 15ml | 150ml |
| 5 * intambwe imwe ivanze | 800μl | 4 * 1ml | 40ml |
| Intambwe imwe ya enzyme ivanze | 200μl | 1ml | 10ml |
| 50 * ROX yerekana irangi 1 | 80μl | 400μl | 4 * 1ml |
| 50 * ROX yerekana irangi 2 | 80μl | 400μl | 4 * 1ml |
a.Buffer Intambwe imwe irimo DNTP ivanze na Mg2 +.
b.Enzyme Ivanga cyane cyane irimo transcript transcript, Hot Start Taq ADN polymerase (guhindura antibody) na inhibitor ya RNase.
c.Byakoreshejwe mugukosora amakosa yibyaha bya fluorescene hagati yamariba atandukanye.
c.ROX: Ugomba guhitamo kalibrasi ukurikije icyitegererezo cyibikoresho byo gupima.
Porogaramu
Kumenya virusi
gusuzuma no gukora ubushakashatsi ku kibyimba
Kumenya indwara zinyamaswa
Kwipimisha hakiri kare indwara zumurage
Kumenya mikorobe itera indwara mubiryo
Kohereza no kubika
Ubwikorezi:Amapaki
Uburyo bwo kubika:Ubike kuri -30 ~ -15 ℃.
Ubuzima bwa Shief:Imyaka 1
