RT-LAMP Fluorescent Master mix (Ifu ya Lyophilized)
Ibisobanuro ku bicuruzwa
LAMP kuri ubu ikoreshwa cyane muburyo bwa tekinoroji ya isothermal amplification.Ikoresha primers 4-6 ishobora kumenya uturere 6 twihariye kuri gen igenewe, kandi ishingiye kubikorwa bikomeye byo kwimura imirongo ya Bst ADN polymerase.Hariho uburyo bwinshi bwo gutahura LAMP, harimo uburyo bwo gusiga irangi, uburyo bwa pH colimetric, uburyo bwo guhungabana, HNB, calcein, nibindi. RT-LAMP ni ubwoko bumwe bwa LAMP reaction hamwe na RNA nkicyitegererezo.RT-LAMP Fluorescent Master mix (Lyophilized Powder) iri muburyo bwa Lyofilized Powder, kandi ikeneye kongeramo primers na templates mugihe uyikoresheje.
Ibisobanuro
| Ibintu byo kwipimisha | Ibisobanuro |
| Endonulease | Ntabwo yatoranijwe |
| Igikorwa cya RNase | Nta n'umwe wamenyekanye |
| Igikorwa cya DNase | Nta n'umwe wamenyekanye |
| Igikorwa cya Nickase | Nta n'umwe wamenyekanye |
| E. coli.gDNA | ≤10kopi / 500U |
Ibigize
Iki gicuruzwa kirimo reaction ya Buffer, RT-Enzymes ivanze ya Bst ADN Polymerase na Thermostable Reverse Transcriptase, Lyoprotectant na Fluorescent Irangi.
Kwinginga
Kwiyongera kwa Isothermal ya ADN na RNA.
Kohereza no Kubika
Ubwikorezi:Ibidukikije
Uburyo bwo kubika:Ubike kuri -20 ℃
Basabwe kongera kwipimisha:Amezi 18
