Hindura Transcriptase, Glycerol-yubusa

Guhindura inyandiko-mvugo 200U / μL, Glycerol-frees ni inyandiko mishya yinyuma yabonetse binyuze mubuhanga bwubuhanga.Ugereranije na M-MLV () Inyuma ya Transcriptase, itekanye yubushyuhe bwayo Iratera imbere cyane kandi irashobora kwihanganira ubushyuhe bwibisubizo bigera kuri 65 ° C, bikwiranye no kwandukura inyandikorugero ya RNA hamwe nuburyo bukomeye bwa kabiri.Muri icyo gihe, enzyme yazamuye ubucuti hamwe nicyitegererezo, ikwiranye no guhinduranya inyandiko mvugo ntoya na kopi nkeya.Ubushobozi bwa Reverse Transcriptase yo guhuza uburebure bwa cDNA nabwo bwaratejwe imbere, kandi cDNA igera kuri 19.8 kb irashobora kongerwa.Reverse Transcriptase, 200 U / μL, Glycerol idafite ibisekuruza bya gatatu thermostable reverse transcriptase (verisiyo ya glycerol) irashobora gukoreshwa mugutegura imyiteguro ya lyofilize, reagent ya RT-LAMP, nibindi.