Intambwe imwe RT-qPCR probe kit
Ibisobanuro
U.Iki gicuruzwa gihuza imikorere isumba iyindi yintambwe yihariye ya Reverse Transcriptase hamwe na Hot-Champagne Taq ADN Polymerase ishyushye, hamwe na bffer yimiti ikonjesha neza, ifite uburyo bwiza bwo kongera imbaraga, kuringaniza no kwihariye, kandi ikaba ihujwe no gukonjesha-gukama bitandukanye. inzira. Mubyongeyeho, sisitemu ya DUTP / UDG yo kurwanya umwanda yinjizwa muri reagent, ishobora gukora ku bushyuhe bwicyumba, ikuraho ingaruka ziterwa n’ibicuruzwa byongera imbaraga kuri qPCR, kandi ikemeza ko ibisubizo ari ukuri.
Amahame shingiro ya RT-qPCR
Ibisobanuro
| Ibizamini | Ibisobanuro | Igisubizo |
| (URUPAPURO RWA SDS) Ubuziranenge bwimigabane ya Enzyme (URUPAPURO RWA SDS) | ≥95% | Pass |
| Endonuc Please Igikorwa | Ntibimenyekana | Pass |
| Igikorwa cyo Gusohora | Ntibimenyekana | Pass |
| Igikorwa cya Rnase | Ntibimenyekana | Pass |
| Ibisigisigi bya E.coli ADN | Op 1copies / 60 | Pass |
| Imikorere-Sisitemu | 90% ≤110% | Pass |
Ibigize
| Ibigize | 100rxns | 1.000 | 5.000 rxns |
| Ubusa ddH2O | 2 * 1ml | 20ml | 100ml |
| 5 * intambwe imwe ivanze | 600μl | 6 * 1ml | 30ml |
| Intambwe imwe ya enzyme ivanze | 150μl | 2 * 750μl | 7.5ml |
| 50 * ROX yerekana irangi 1 | 60μl | 600μl | 3 * 1ml |
| 50 * ROX yerekana irangi 2 | 60μl | 600μl | 3 * 1ml |
a.Buffer Intambwe imwe irimo DNTP ivanze na Mg2 +.
b.Enzyme ivanze ahanini irimo revers
transcriptase, Hot Start Taq ADN polymerase (guhindura antibody) na inhibitor ya RNase.
c.Byakoreshejwe mugukosora amakosa yibyaha bya fluorescene hagati yamariba atandukanye.
c.ROX: Ugomba guhitamo kalibrasi ukurikije icyitegererezo cyibikoresho byo gupima.
Porogaramu
Kumenya QPCR
Kohereza no kubika
Ubwikorezi:Amapaki
Uburyo bwo kubika:Ubike kuri -20 ℃.
Ubuzima bwa Shief:Amezi 18
