Recombinant Deoxyribonuclease I (DNase I, RNase-yubusa)
Ibisobanuro
Recombinant Deoxyribonuclease I, ni endonuclease ishobora gusya ADN imwe imwe cyangwa ebyiri.Ihindura hydrolyzes ya fosifori kugirango itange mono- na oligodeoxynucleotide irimo 5'-fosifate nitsinda rya 3'-OH.Uburyo bwiza bwo gukora pH urwego rwa DNase I ni 7-8.Igikorwa cya DNase I giterwa na Ca2 + kandi gishobora gukoreshwa na ion zingana nicyuma nka Co2 +, Mn2 +, Zn2 +, nibindi imbere ya Mg2 +, DNase nshobora guhita nkata ADN ebyiri zibiri kurubuga urwo arirwo rwose;mugihe imbere ya Mn2 +, DNase nshobora guca ADN inshuro ebyiri kumurongo umwe, nkora impera zidafite ishingiro cyangwa nuclei 1-2 Sticky irangirana na nucleotide irenze.Iyi misemburo ikomoka kuri recombinant E. coli, ntabwo irimo RNase, kandi irashobora gukoreshwa mugutunganya ingero zitandukanye za RNA
Imiterere yimiti
Porogaramu
Gukuramo RNA: gutegura RNA idafite ADN;
Nyuma yo kwandukura vitro hamwe na RNA polymerase, nka T7 RNA Polymerase (Cat # 10618), ikoreshwa mugukuraho inyandikorugero ADN;
Tegura RNA idafite ADN mbere ya RT-PCR na RT-qPCR;
Yifashishijwe ifatanije na ADN Polymerase I (Injangwe # 12903) kugirango yandike ADN ibisobanuro byizina;
Ikoreshwa mu gusesengura ibirenge kugirango isesengure imikoranire ya ADN na poroteyine;
Gukora isomero ryibice bidasanzwe;
Muri TUNEL itahura apoptose, ADN ya genomic yogoshe igice yakoreshejwe nkigenzura ryiza.
Kohereza no kubika
Ubwikorezi:Amapaki
Uburyo bwo kubika:Ubike muri -20 ℃
Ubuzima bwa Shelf:Imyaka 2
Inyandiko:
1.Enzymes igomba kubikwa mu isanduku ya barafu cyangwa ku bwogero bwa barafu iyo ikoreshejwe, kandi igomba kubikwa kuri -20 ° C ako kanya nyuma yo kuyikoresha.
2.Ku mutekano wawe nubuzima bwawe, nyamuneka wambare ibikoresho birinda umuntu (PPE), nk'amakoti ya laboratoire hamwe na gants imwe ikoreshwa, mugihe ukorana niki gicuruzwa.
