Intambwe imwe RT-qPCR Probe Kit (KUBUNTU)
Ibisobanuro
Intambwe imwe RT-qPCR Probe Kit (KUBERA VUBA) nigikoresho gishingiye kuri RT-qPCR cyihuta cyerekana ibikoresho bikwiranye na PCR imwe cyangwa plex nyinshi igereranya PCR ikoresheje RNA nk'icyitegererezo (nka virusi ya RNA).Iki gicuruzwa gikoresha igisekuru gishya cya antibody yahinduwe na Taq ADN Polymerase hamwe nintambwe imwe yihariye ya Reverse Transcriptase, hamwe na buffer nziza kugirango yongerwe vuba, ifite umuvuduko mwinshi wo kwihuta, gukora neza kandi neza.Ifasha kuringaniza amplifisione murwego rumwe-plex na byinshi-plex byintangarugero ntoya kandi murwego rwo hejuru mugihe gito.
Inzira yo Kwitabira
Ibigize
| Ibigize | 200rxns | 1.000 | 10,000 rxns |
| Ubusa ddH2O | 3 * 1ml | 15ml | 150ml |
| 5 * intambwe imwe ivanze | 800μl | 4 * 1ml | 40ml |
| Intambwe imwe ya enzyme ivanze | 200μl | 1ml | 10ml |
| 50 * ROX yerekana irangi 1 | 80μl | 400μl | 4 * 1ml |
| 50 * ROX yerekana irangi 2 | 80μl | 400μl | 4 * 1ml |
a.Buffer Intambwe imwe irimo DNTP ivanze na Mg2 +.
b.Enzyme ivanze ahanini irimo revers
transcriptase, Hot Start Taq ADN polymerase (guhindura antibody) na inhibitor ya RNase.
c.Byakoreshejwe mugukosora amakosa yibyaha bya fluorescene hagati yamariba atandukanye.
c.ROX: Ugomba guhitamo kalibrasi ukurikije icyitegererezo cyibikoresho byo gupima.
Porogaramu
Kumenya virusi
gusuzuma no gukora ubushakashatsi ku kibyimba
Kumenya indwara zinyamaswa
Kwipimisha hakiri kare indwara zumurage
Kumenya mikorobe itera indwara mubiryo
Kohereza no kubika
Ubwikorezi:Amapaki
Uburyo bwo kubika:Ubike kuri -30 ~ -15 ℃.
Ubuzima bwa Shief:Imyaka 1
