RT-LAMP Fluorescent (Umupira wa Lyophilized)
RT-LAMP Fluorescent Master Mix (Powder ya Lyophilized) ikubiyemo buffer reaction, Bst ADN polymerase na Bst ADN polymerase hamwe nubushyuhe bwo kurwanya ubushyuhe, kurinda lyofilize, hamwe n irangi rya fluorescent.Igicuruzwa kiri muburyo bwamasaro ya lyofilize, kandi primers na templates gusa bigomba kongerwaho kugirango bikoreshwe.
Ibigize ibintu
RT-LAMP Fluorescent Master mix (Ifu ya Lyophilized)
Ikoreshwa
Kuri ADN cyangwa RNA isothermal amplification
Ububiko
Gutwara no kubika kuri 2 ~ 8 ° C.
Ubuzima bwa Shelf: amezi 12.
Porotokole
1. Kuramo umubare uhuye namasaro ya lyofilize ukurikije umubare wibizamini.
2. Gutegura sisitemu yo kwitwara
| Ibigize | Umubare |
| RT-LAMP Fluorescent Master mix (Ifu ya Lyophilized) | Amasaro 2 |
| Irangi rya Fluorescent | x μL |
| 10 mix Primer Kuvanga a | 5 μL |
| Inyandikorugero ADN / RNA b | n μL |
| Igiteranyo | 50 μL |
Inyandiko:
1) a.10 × Primer mix: 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Umuzingi F / B;
2) b.Icyitegererezo cya acide nucleic kirasabwa gushonga mumazi ya DEPC.
Hyasen Biotechnology Co, Ltd.
3) Gahunda yo Kwitabira
| Ubushyuhe | Igihe | Amagare |
| 65 ℃ | 60s a | 30 |
Inyandiko:
1. Kusanya ikimenyetso cya fluorescent
Inyandiko
1. Ubushyuhe bwa reaction burashobora kuba bwiza hagati ya 62 ° C na 68 ° C ukurikije primerimiterere;
2. Ubushakashatsi bugomba guhuza imikorere, harimo gutegura sisitemu ya reaction,icyitegererezo cyo gutunganya no kunywa;
3. Kugira ngo wirinde kwanduza, birasabwa gutegura sisitemu yo kwitwara mumeza ya ultra-isukuhanyuma ongeraho inyandikorugero kuri fume hood mubindi byumba kugirango wirinde kwivanga kwiza.
