Bst 2.0 ADN Polymerase (Glycerol yubusa, ubwinshi)
Bst ADN polymerase V2 yakomotse kuri Bacillus stearothermophilus ADN Polymerase I, ifite 5 ′ → 3 ′ ibikorwa bya polymerase ya ADN nigikorwa gikomeye cyo gusimbuza urunigi, ariko nta gikorwa cya 5 ′ → 3 ′.Bst ADN Polymerase V2 nibyiza cyane muburyo bwo kwimura imirongo, kwimura isothermal amplification LAMP (Loop mediated isothermal amplification) hamwe nuburyo bwihuse.Iyi Bst DNA polymerase V2 ishoboye guhagarika ibikorwa bya ADN polymerase mubushyuhe bwicyumba, kugirango ikorwe kandi sisitemu yo kubyitwaramo irashobora gutegurwa mubushyuhe bwicyumba, ikarinda amplificatif idasanzwe kandi ikanoza imikorere yibikorwa, kandi iyi verisiyo irashobora be lyofilized.Mubyongeyeho, irashoboye kurekura ibikorwa byayo mubushyuhe bwo hejuru, bityo bikuraho gukenera intambwe itandukanye.
Ibigize
| Ibigize | HC5007A-01 | HC5007A-02 | HC5007A-03 |
| Bst ADN polymerase V2 (Glycerol idafite) (32U / μL) | 0.05 mL | 0,25 mL | 2.5 mL |
| 10 × HC Bst V2 Buffer | 1.5 mL | 2 × 1.5 mL | 3 × 10 mL |
| MgSO4 (100mM) | 1.5 mL | 2 × 1.5 mL | 2 × 10 mL |
Porogaramu
1.LAMP isothermal amplification
2.ADN umurongo umwe wimurwa
3.Urwego rwo hejuru rwa GC
4.Urutonde rwa ADN urwego rwa nanogram.
Imiterere y'Ububiko
Ubwikorezi buri munsi ya 0 ° C kandi bubikwa kuri -25 ° C ~ -15 ° C.
Igisobanuro
Igice kimwe gisobanurwa nkubunini bwa enzyme yinjiza nmol 25 ya dNTP mubintu bitangirika aside muminota 30 kuri 65 ° C.
Amatara
| Ibigize | 25 μLSisitemu |
| 10 × HC Bst V2 Buffer | 2.5 μL |
| MgSO4 (100mM) | 1.5 μL |
| DNTPs (10mM buri umwe) | 3.5 μL |
| SYTO ™ 16 Icyatsi (25 ×)a | 1.0 μL |
| Kuvanga primerb | 6 μL |
| Bst ADN Polymerase V2 (Glycerol idafite) (32 U / uL) | 0,25 μL |
| Inyandikorugero | × μL |
| ddH₂O | Kugera kuri 25 μL |
Inyandiko:
1) a.SYTOTM 16 Icyatsi (25 ×): Ukurikije ubushakashatsi bukenewe, andi marangi arashobora gukoreshwa nkibisimburwa;
2) b.Kuvanga primer: byabonetse mukuvanga 20 µM FIP, 20 µM BIP, 2.5 µM F3, 2.5 µM B3, 5 µM LF, 5 µM LB nibindi bitabo.
Imyitwarire n'imiterere
1 × HC Bst V2 Buffer, ubushyuhe bwa incubation buri hagati ya 60 ° C na 65 ° C.
Gushyushya
80 ° C, 20min.
