ELEISA KIT ya Trypsin

Recombinant Trypsin ikoreshwa kenshi mubikorwa bya biofarmaceutical-mugihe cyo gutegura selile cyangwa muguhindura no gukora ibicuruzwa.Trypsin itera ingaruka z'umutekano bityo igomba gukurwaho mbere yo gusohora ibicuruzwa byanyuma.Iyi Sandwich Kit ni igipimo cyo gupima ingano ya Trypsin isigaye mu muco w’akagari nizindi nzira mubikorwa bya biofarmaceutical mugihe Trypsin ikoreshwa.

Iki gikoresho ni Enzyme-ihuza Immunosorbent Assay (ELISA).Isahani yashizwemo mbere na antibody ya Porcine trypsin.Trypsin igaragara muri sample yongeyeho kandi ihuza antibodies zometse ku mariba.Hanyuma biotinylated Porcine trypsin antibody yongeweho kandi ihuza na trypsin murugero.Nyuma yo gukaraba, HRP-Streptavidin yongeweho kandi ihuza antibody ya Biotinylated trypsin.Nyuma ya incubation idahuza HRP-Streptavidin yogejwe.Noneho igisubizo cya TMB substrate cyongeweho kandi kigatangizwa na HRP kugirango gitange ibicuruzwa byamabara yubururu byahindutse umuhondo nyuma yo kongeramo aside ihagarika.Ubucucike bwumuhondo buringaniye nintego ya trypsin
icyitegererezo cyafashwe mu isahani.Kwinjira byapimwe kuri 450 nm.