Cholesterol oxyde (COD / CHOD)
Ibisobanuro
Cholesterol oxydease (CHOD) itera intambwe yambere muri cholesterol catabolism.Bagiteri zimwe na zimwe zidatera indwara, nka Streptomyces zirashobora gukoresha cholesterol nkisoko ya karubone.Indwara ya bagiteri itera indwara, nka Rhodococcus equi, isaba CHOD kwanduza macrophage ya nyirarureshwa. CHOD ikora neza. Cholesterol yabanje guhumeka kuri cholest-5-en-3-imwe mu ntambwe isaba FAD.Cholest-5-en-3-imwe isomerized to cholest-4-en3-one.Isomerisation reaction irashobora guhinduka igice.Igikorwa cya CHOD giterwa nibintu bifatika bya membrane bihujwe na substrate.
CHOD ikoreshwa mukumenya cholesterol ya serumu.Nibintu bya kabiri bikoreshwa cyane muri enzyme mugusuzuma nyuma ya glucose oxyde.CHOD isanga kandi ikoreshwa muri microanalyse ya steroyide mu byitegererezo by'ibiribwa no mu gutandukanya 3-ketosteroide na 3b-hydroxysteroide.Ibimera bya transgenji byerekana okiside ya cholesterol birakorwaho iperereza mu kurwanya ipamba boll weevil.Cholesterol oxydease nayo yakoreshejwe nka probe ya molekulari kugirango isobanure imiterere ya selile.
Imiterere yimiti
Ihame ry'imyitwarire
Cholesterol + O2 →△4-Cholesten-3-imwe + H2O2
Ibisobanuro
| Ibizamini | Ibisobanuro |
| Ibisobanuro | Ifu ya amorphous yumuhondo, lyofilize |
| Igikorwa | ≥8U / mg |
| Isuku (SDS-PAGE) | ≥90% |
| Gukemura (10mg ifu / ml) | Biragaragara |
| Catalase | ≤0.001% |
| Glucose oxyde | ≤0.01% |
| Cholesterol esterase | ≤0.01% |
| ATPase | ≤0.005% |
Gutwara no kubika
Ubwikorezi:Yoherejwe munsi ya -15 ° C.
Ububiko:Ubike kuri -25 ~ -15 ° C (Igihe kirekire), 2-8 ° C (Igihe gito)
Basabwe kongera gukora ikizaminiUbuzima:Umwaka 1
