D-Lactate dehydrogenase (LDH)
Ibisobanuro
Enzyme ikoreshwa mugukuraho pyruvate mubyemezo ikorana na NADH (triglyceride, lipase, aldolase, aspartate aminotransferase, glutamate dehydrogenase).
Ikoreshwa
1.Alkaline fosifata ihujwe na poroteyine (antibodies, streptavidin nibindi,) irashobora kumenya neza molekile zigenewe, kandi irashobora gukoreshwa muri ELISA, WB no kumenya amateka ya histochemiki;
2.Alkaline fosifata irashobora gukoreshwa mugutandukanya 5 '-imiterere ya ADN cyangwa RNA kugirango wirinde kwihuza;
3.A ADN ya dephosifora yavuzwe haruguru cyangwa RNA irashobora gushyirwaho ikimenyetso cya fosifeti ya radio (binyuze kuri T4 poly-nucleotide kinase)
Imiterere yimiti
Uburyo bwo kubyitwaramo
D-Amashanyarazi + NAD+→ Pyruvate + NADH + H.+
Ibisobanuro
| Ibizamini | Ibisobanuro |
| Ibisobanuro | Ifu ya amorphous yera, lyofilize |
| Igikorwa | ≥200U / mg |
| Isuku (SDS-PAGE) | ≥90% |
| Gukemura (10mg ifu / mL) | Biragaragara |
| NADH / NADPH oxyde | ≤0.01% |
| Malate dehydrogenase | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| Glutamate Dehydrogenase | ≤0.003% |
| Gutandukanya aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Gutwara no kubika
Ubwikorezi:AmbIent
Ububiko:Ubike kuri -20 ° C (Igihe kirekire), 2-8 ° C (igihe gito)
Basabwe kongera gukora ikizaminiUbuzima:Imyaka 2
