Glucose Dehydrogenase (GDH)
Ibisobanuro
Glutamate Dehydrogenase (GDH) ni enzyme ya mitochondrial itanga umusemburo wa okiside uhindagurika wa glutamate kuri a-ketoglutarate kandi ikora nk'isano nyamukuru hagati yinzira ya anabolike na catabolika.Mu nyamaswa z’inyamabere, GDH igengwa na allosteric kandi ifite ibikorwa byinshi mu mwijima, impyiko, ubwonko, na pancreas.Igikorwa cya GDH muri serumu kirashobora gukoreshwa mugutandukanya indwara zumwijima bitewe no gutwika umwijima, zitagaragaza ibikorwa bya serumu ya GDH hejuru, nindwara zitera hepatocyte necrosis, bikaviramo serumu GDH.
Igikorwa cya GDH kigenwa na enzyme ihujwe hamwe glutamate ikoreshwa na GDH itanga NADH, ikora hamwe na probe itanga ibicuruzwa byamabara (450 nm) bijyanye nibikorwa bya GDH bihari.Igice kimwe cya GDH ni ingano ya enzyme izabyara 1.0 mmole ya NADH kumunota kuri pH 7.6 kuri 37 ° C.
Imiterere yimiti
Uburyo bwo kubyitwaramo
D-Glucose + uwakiriye → D-Glucono-1,5-lactone + yagabanije kwakira
Ibisobanuro
| Ibizamini | Ibisobanuro |
| Ibisobanuro | Ifu ya amorphous yera, lyofilize |
| Igikorwa | ≥160U / mg |
| Isuku (SDS-PAGE) | ≥90% |
| Gukemura (10 mg ifu / mL) | Biragaragara |
| Kwanduza imisemburo | |
| Glucose dehydrogenase (NAD) | ≤0.02% |
| Hexokinase | ≤0.02% |
| A-Glucosidase | ≤0.02% |
Gutwara no kubika
Ubwikorezi: Amapaki
Ububiko:Ubike kuri -25 ~ -15 ° C (Igihe kirekire), 2-8 ° C (igihe gito)
Basabwe kongera gukora ikizaminiUbuzima: Amezi 18
