Uracil ADN Glycoylase (Glycerol-idafite)
Ibisobanuro
Thermosensitive UDG (uracil-ADN glycosylase) irashobora guhagarika hydrolysis ya base ya uracil yumurongo wa ADN irimo uracil hamwe na N-glycosidic ihuza isukari-fosifate yumugongo kugirango irekure uracil yubusa.Ugereranije n’imisemburo isanzwe ya UDG, imisemburo ya UDG yumuriro irinda ibikorwa bisigaye byimisemburo isanzwe ya UDG nyuma yo kudakora, bishobora gutesha agaciro dU irimo ibicuruzwa byongera ingufu mubushyuhe bwicyumba.Iki gicuruzwa gikora mubushyuhe bwicyumba kandi cyumva ubushyuhe kandi gikunda kudakora.
Imiterere yimiti
Ibisobanuro
Enzyme | Glycosylase |
Buffer | Ububiko |
Gushyushya | 50 ° C, iminota 10 |
Igisobanuro | Igice kimwe (U) gisobanurwa nkubunini bwa enzyme isabwa kugirango uhagarike hydrolysis ya 1 μg dU irimo dsDNA muminota 30 kuri 25 ° C. |
Porogaramu
Kuraho dU irimo PCR ibicuruzwa byanduye aerosol.
Gukuraho ibice bya uracil muri ADN imwe cyangwa imirongo ibiri
Kohereza no kubika
Ubwikorezi:Amapaki
Uburyo bwo kubika:Ubike kuri -15 ℃ ~ -25 ℃
Ubuzima bwa Shief:1years