Proteinase K (Ifu ya Lyophile)
Ibyiza
Stability Ibikorwa bihamye hamwe nibikorwa bya enzyme bishingiye ku buhanga bwihindagurika
● Kwihanganira umunyu wa Guanidine
● RNase kubuntu, DNase kubuntu na Nickase kubuntu, ADN <5 pg / mg
Ibisobanuro
Proteinase K ni proteine ihamye hamwe na substrate yagutse.Itesha agaciro poroteyine nyinshi muri kavukire ndetse no imbere yimyenda.Ibimenyetso bivuye mubushakashatsi bwa kristu na molekuline byerekana ko enzyme ari iyumuryango wa subtilisin hamwe na site ikora catalitike triad (Asp 39-His 69-Ser 224).Ikibanza cyiganjemo clavage ni peptide ihuza peptide yegeranye na carboxyl groupe ya aliphatic na aromatic amino acide hamwe na alpha amino yahagaritswe.Bikunze gukoreshwa muburyo bwagutse.
Imiterere yimiti
Ibisobanuro
Ibintu byo kwipimisha | Ibisobanuro |
Ibisobanuro | Umweru kugeza ifu ya amorphous yera, Lyophilied |
Igikorwa | ≥30U / mg |
Gukemura (50mg Ifu / mL) | Biragaragara |
RNase | Nta n'umwe wamenyekanye |
DNase | Nta n'umwe wamenyekanye |
Nickase | Nta n'umwe wamenyekanye |
Porogaramu
Ibikoresho byo gusuzuma indwara;
Ibikoresho byo gukuramo RNA na ADN;
Gukuramo ibice bitari poroteyine biva mu ngingo, kwangirika kwa poroteyine, nka
Inkingo za ADN no gutegura heparin;
Gutegura ADN ya chromosome na pulsed electrophoreis;
Iburengerazuba;
Enzymatique glycosylated albumin reagents mugupima vitro
Kohereza no kubika
Kohereza:Ibidukikije
Uburyo bwo kubika:Ubike kuri -20 ℃ (Igihe kirekire) / 2-8 ℃ (Igihe gito)
Basabwe kongera kwipimisha:Imyaka
Kwirinda
Wambare uturindantoki two kurinda hamwe na gogles mugihe ukoresha cyangwa upima, kandi ukomeze guhumeka neza nyuma yo kubikoresha.Iki gicuruzwa gishobora gutera allergie reaction.Tera uburakari bukabije bw'amaso.Niba ihumeka, irashobora gutera allergie cyangwa ibimenyetso bya asima cyangwa dyspnea.Birashobora gutera uburakari.
Suzuma Igice
Igice kimwe (U) gisobanurwa nkubunini bwa enzyme isabwa kuri hydrolyze casein kugirango itange 1 μ mol tyrosine kumunota mubihe bikurikira.