Intambwe imwe RT-qPCR probe kit

Intambwe imwe qRT-PCR Probe Kit yagenewe byumwihariko kuri qPCR ikoresha mu buryo butaziguye RNA (urugero virusi RNA) nk'icyitegererezo.Ukoresheje primers yihariye (GSP), transcription transcription na qPCR birashobora kurangirira mumiyoboro imwe, bikagabanya cyane uburyo bwo kuvoma no guhura nubwandu.Irashobora kudakorwa kuri 55 ℃ itagize ingaruka kumikorere no kumva neza qRT-PCR.Uhujije imikorere isumba iyindi ya HiScript III Reverse Transcriptase hamwe na Hot-Champagne Taq ADN Polymerase ishyushye, hamwe na sisitemu yogukoresha neza, sensibilité yo kumenya intambwe imwe qRT-PCR Probe Kit irashobora kugera kuri 0.1 pg ya RNA yose cyangwa munsi ya kopi 10 za RNA yerekana kandi ifite ubushyuhe buhanitse.Intambwe imwe qRT-PCR Probe Kit itangwa muri Master mix.5 × Intambwe imwe ivanze ikubiyemo buffer nziza na DNTP / dUTP ivanze, kandi irakwiriye kuri sisitemu yihariye yo gutahura ishingiye kuri fluorescence yanditseho probe (urugero: TaqMan).