Intambwe imwe RT-qPCR SYBR Icyatsi kibisi

Universal Blue qPCR Master mix (Irangi rishingiye) nigisubizo kibanziriza 2 × igihe nyacyo cyo kugereranya PCR amplification irangwa no kwiyumvamo ibintu byinshi kandi byihariye, ni ubururu mu ibara, kandi bifite ingaruka zo gushakisha icyitegererezo.Ibyingenzi bigize Taq ADN polymerase ikoresha antibody ishyushye itangira kugirango ibuze neza amplification idasanzwe bitewe na primer annealing mugihe cyo gutegura icyitegererezo.Muri icyo gihe, formula yongeramo ibintu biteza imbere kunoza imikorere ya PCR no kuringaniza amplifisione ya gen hamwe nibintu bitandukanye bya GC (30 ~ 70%), kugirango PCR yuzuye ibone umubano mwiza wumurongo muburyo bwinshi. karere.Ibicuruzwa birimo irangi ryihariye rya ROX Passive Reference Irangi, rikoreshwa mubikoresho byose bya qPCR.Ntabwo ari ngombwa guhindura concentration ya ROX kubikoresho bitandukanye.Birakenewe gusa kongeramo primers na templates kugirango utegure sisitemu yo kwitwara kugirango amplification.