Rnase Inhibitor

Injangwe No: HC2010A

Ipaki: 2KU / 10KU / 100KU

Murine RNase inhibitor ni recombinant murine RNase inhibitor yagaragajwe kandi yeza kuri E.coli.

Ibisobanuro ku bicuruzwa

Ibisobanuro birambuye

Murine RNase inhibitor ni recombinant murine RNase inhibitor yagaragajwe kandi yeza kuri E.coli.Ihuza na RNase A, B cyangwa C mu kigereranyo cya 1: 1 binyuze mu guhuza kudahuza, bityo bikabuza ibikorwa byimisemburo itatu kandi bikarinda RNA kwangirika.Ariko, Ntabwo ari byiza kurwanya RNase 1, RNase T1, S1 Nuclease, RNase H cyangwa RNase kuva Aspergillus.Inhibitor ya Murine RNase yageragejwe na RT-PCR, RT-qPCR na IVT mRNA, kandi yari ihujwe n’ubucuruzi butandukanye bwa Reverse transcriptase, ADN polymerase na polymerase ya RNA.

Ugereranije na RNase inhibitor ya muntu, murine RNase inhibitor ntabwo irimo sisitemu ebyiri zumva cyane okiside itera kudakora kwa inhibitor.Ibyo bituma bihagarara neza kuri DTT (munsi ya mM 1).Iyi mikorere ituma ikwiriye gukoreshwa mubitekerezo aho kwibanda cyane kwa DTT bihabanye nigisubizo (urugero Real-time RT-PCR).

Mbere: Hotstart Taq ADN Polymerase HC1012A

Ibikurikira: Hs Taq ADN Polymerase (Glycerol-idafite)

Agusaba

Ibicuruzwa birashobora gukoreshwa cyane mubigeragezo ibyo aribyo byose aho kwivanga kwa RNase bishoboka kugirango wirinde kwangirika kwa RNA, nka:

1.Synthesis yumurongo wambere wa cDNA, RT-PCR, RT-qPCR, nibindi.

2.Irinda RNA kwangirika mugihe muri vitro transcription / ibisobanuro (urugero, muri sisitemu yo kwigana virusi ya vitro).

3.Kubuza ibikorwa bya RNase mugihe cyo gutandukanya RNA no kwezwa.

Ububiko

Ibicuruzwa birashobora kubikwa kuri -25 ～ - 15 ℃, byemewe kumyaka 2.

Ububiko

50 mM KCl, 20 mM HEPES-KOH (pH 7,6, 25 ℃), 8 mM DTT na 50% glycerol.

Igisobanuro cyibice

Ingano ya murine RNase inhibitor isabwa kugirango ihagarike ibikorwa bya 5ng ya ribonuclease A kuri 50% yasobanuwe nkigice kimwe (U).

Uburemere bwa molekuline ya poroteyine

Uburemere bwa molekuline ya murine RNase inhibitor ni 50 kDa.

Kugenzura ubuziranenge

Exonuc Please Igikorwa:

40 U ya murine RNase inhibitor hamwe na 1 μg λ -Hind III ya ADN igogora ADN kuri 37 ℃ kumasaha 16 itanga nta kwangirika nkuko byagenwe na agarose gel electrophorei.
Igikorwa cya Endonuc Please:

40 U ya murine RNase inhibitor hamwe na 1μ g λ ADN kuri 37 ℃ kumasaha 16 nta musaruro utanga nkuko byagenwe na agarose gel electrophorei.

Nicking Igikorwa:

40U ya murine RNase inhibitor hamwe na 1μ g pBR322 kuri 37 ℃ kumasaha 16 nta musaruro utanga nkuko byagenwe na agarose gel electrophorei.

RNase Igikorwa:

40U ya murine RNase inhibitor hamwe na 1,6μ g g MS2 RNA kuri 4h kuri 37 ℃ nta musaruro ugabanuka nkuko byagenwe na agarose gel electrophorei.

E.ADN ya coli:

40 U ya murine RNase inhibitor isuzumwa kugirango ADN genomic E. coli ikoreshe TaqMan qPCR hamwe na primers yihariye ya E. coli 16S rRNA.E. coli genomic ADN yanduye ni ≤ 0.1 pg / 40 U.

Notes

1.Ihungabana rikabije cyangwa gukurura bizatuma enzyme idakora.

2.Ubushyuhe bwiza buringaniye bwibi inhibitor yari 25-55 ℃, kandi ntiyakorewe kuri 65 ℃ no hejuru.

3.Ibikorwa bya RNase H, RNase 1 na RNase T1 ntibyabujijwe na murine RNase inhibitor.

4.Kubuza ibikorwa bya RNase byabonetse mubice byinshi bya pH (pH 5-9 byose byakoraga), kandi ibikorwa byinshi byagaragaye kuri pH 7-8.

5.Kubera ko ribonuclease isanzwe igumana ibikorwa mugihe cyo gutandukanya ibintu, hagomba kwitonderwa kugirango wirinde kwanga molekile ya RNase Inhibitor yahujwe na ribonuclease.Kugira ngo wirinde kurekura ribonuclease ikora, ubushyuhe burenze 50 ° C hamwe n’ubushyuhe bwinshi bwa urea cyangwa ibindi bikoresho byerekana.