M-MLV Guhindura Inyandiko (Glycerol kubuntu)
Lyophilizable Reverse Transcriptase.Irashobora gukoreshwa muburyo bwa tekinoroji ya lyophilisation mugihe ikomeza ibikorwa bikomeye byo kwandukura no gutuza.Ibicuruzwa ntabwo birimo ibicuruzwa, nyamuneka ongeraho ibyawe nkuko bikenewe.
Ibigize
| Ibigize | HC2005A-01 (10,000U) | HC2005A-02 (40.000U) |
| Hindura Transcriptase (Ubuntu bwa Glycerol) (200U / μL) | 50 μL | 200 μL |
| 5 × Buffer | 200 μL | 800 μL |
Gusaba:
Irakoreshwa kumurongo umwe RT-qPCR.
Imiterere y'Ububiko
Ubike kuri -30 ~ -15 ° C no gutwara kuri ≤0 ° C.
Igisobanuro
Igice kimwe (U) gisobanurwa nkubunini bwa enzyme yinjizamo 1 nmol ya dTTP mubintu bya aside-idashobora gushonga muri 10min kuri 37 ° C, hamwe na Poly (rA) · Oligo (dT) nkicyitegererezo / primer.
Inyandiko
Kubushakashatsi ukoreshe gusa.Ntabwo ari ugukoresha muburyo bwo gusuzuma.
1.Nyamuneka saba ahantu ho kugerageza;Kwambara uturindantoki twajugunywe hamwe na masike;Koresha RNase itagikoreshwa nka centrifuge tubes hamwe ninama za pipette.
2.Gumana RNA kurubura kugirango wirinde kwangirika.
3.Inyandikorugero nziza ya RNA irasabwa kugera kubikorwa byiza byo kwandukura.
