Ultra Nuclease

UltraNuclease ni genetiki ya injeneredendonuclaese ikomoka kuri Serratia marcescens, ishoboye gutesha agaciro ADN cyangwa RNA, haba inshuro ebyiri cyangwa imwe ihagaze, umurongo cyangwa umuzenguruko muburyo butandukanye, yangiza rwose aside nucleic muri 5'-monophosphate oligonucleotide ifite uburebure bwa 3-5base .Nyuma yo guhindura ingengabihe ya geneti, ibibyara byasembuwe, bigaragazwa, kandi bisukurwa na Escherichia coli (E. coli), bigabanya ubukana bwa selile ndengakamere na selile lysate ubushakashatsi bwa siyansi, ariko bikanatezimbere uburyo bwo kweza no gukora ubushakashatsi bwa poroteyine.Irashobora kandi gukoreshwa mubuvuzi bwa gene, kweza virusi, gutanga urukingo, uruganda rwa farumasi ya protein na polysaccharide nkumusemburo wa nucleic aside ukuraho reagent.

Ibiranga ibicuruzwa

Imiterere yo kubika

Transport0 ° C ubwikorezi ； -25 ~ -15 ° C Ububiko years Imyaka 2 ifite agaciro (irinde gukonjesha).

Igisobanuro

Ingano ya enzyme yakoreshejwe muguhindura agaciro ka △ A260 kuri 1.0 muri 30min kuri 37 ° C, pH 8.0, bihwanye na 37μg intanga ngabo ya salmon ADN ikata muri oligonucleotide, yasobanuwe nkigice gikora (U).

Kugenzura ubuziranenge

Intungamubiri zisigaye-selile: ELISA kit

•Kurinda Ibisigisigi: 250KU / mL UltraNuclease yakiriye hamwe na substrate ya 60min, nta gikorwa cyagaragaye.

•Indwara ya bagiteri: LAL-Ikizamini, Pharmacopoeia yo muri Repubulika yUbushinwa Umubumbe wa 4 (2020 Edition) uburyo bwo gupima geli.Amategeko rusange (1143).

•Bioburden: Pharmacopoeia yo muri Repubulika y’Ubushinwa Umubumbe wa 4 (2020 Edition) - Rusange

Amategeko yo Kwipimisha (1101), PRC yigihugu, GB 4789.2-2016.

•Icyuma kiremereye:ICP-AES, HJ776-2015.

Igikorwa

Ibikorwa bya UltraNuclease byabujijwe cyane mugihe SDS yibanze hejuru ya 0.1% cyangwa EDTA

kwibandaho byari hejuru ya 1mM.Surfactant Triton X- 100, Hagati ya 20 na Tween 80 ntacyo byagize kuri nuclease

imitungo iyo kwibandaho byari munsi ya 1.5%.

 Imikoreshereze n'imikoreshereze

• Kuraho acide nucleic acide yibicuruzwa byinkingo, gabanya ibyago byuburozi bwa nucleic aside isigaye kandi bitezimbere umutekano wibicuruzwa.

• Kugabanya ubwiza bwamazi yibiryo biterwa na acide nucleic, kugabanya igihe cyo gutunganya no kongera umusaruro wa proteine.

• Kuraho aside nucleic yapfunyitse ibice (virusi, umubiri ushiramo, nibindi), bifasha

kurekura no kweza ibice.

• Kuvura nuclease birashobora kunoza gukemura no kugarura icyitegererezo cya chromatografiya, electrophoreis hamwe nisesengura rya blotting.

• Mu kuvura gene, aside nucleic ikurwaho kugirango ibone virusi zifitanye isano na adeno.