Superstart qPCR Premix wongeyeho-UNG

Injangwe No: HCB5071E

Superstart qPCR Premix yongeyeho-UNG ni reagent yihariye yagenewe igihe nyacyo PCR yujuje ubuziranenge kandi igereranya ukoresheje iperereza rishingiye ku iperereza, ryakozwe cyane cyane mubikorwa bya lyophilisation.Irimo enzyme ishyushye-itangira enzyme Hotstart Taq plus (DG), ifite ibikorwa byayo bya Taq enzyme bifunze mubushyuhe bwicyumba, bikabuza neza amplification idasanzwe iterwa na primer idasanzwe idasanzwe ya annealing cyangwa primer dimer imiterere yubushyuhe buke, bityo igatera imbere umwihariko wa reaction ya amplification reaction.Iyi reagent ikoresha uburyo bwiza bwa qPCR bwihariye na UNG / dUTP sisitemu yo kurwanya umwanda kugirango igere ku bushyuhe bwihuse, iteza imbere imikorere nubukangurambaga bwa qPCR.Irashobora kubona umurongo mwiza usanzwe mubice byinshi byumubare kandi igakora neza umubare, ikarinda neza amplifisione yibinyoma iterwa nibicuruzwa bya PCR bisigaye cyangwa kwanduza aerosol.Iyi reagent ihujwe nibikoresho byinshi bya fluorescent PCR yibikoresho biva mubakora nka Applied Biosystems, Eppendorf, Bio- Rad na Roche nibindi, kandi byerekana ituze ryiza muburyo bwa lyofilize.

2. Kopi yumubare wintego zikubiye mubwoko butandukanye bw'inyandiko ziratandukanye;nibiba ngombwa, buhoro buhoro dilution irashobora gukorwa kugirango hamenyekane icyitegererezo cyiza cyongeweho.

3. Sisitemu irashobora gukoreshwa neza;mugihe abakiriya bakoresha iyi sisitemu batabanje gukonjesha-gukama, 4 × lyoprotectant irashobora kongerwaho guhitamo; niba hari ibicuruzwa byumye byumye bisabwa, mugihe cya reagent ya reagent icyiciro cyibicuruzwa byemewe, igomba kongeramo 4 × lyoprotectant kugirango ihuze nibice bya sisitemu ya lyofilique. n'ingaruka.

2. Ongeramo icyitegererezo cya acide nucleic kuri poro ya lyofilize hanyuma wongere amazi kugeza kuri 25µL;

3. Kuvanga neza na centrifugation hanyuma ukore kuri mashini.

 Kugenzura ubuziranenge:

1. Kwipimisha kumikorere: sensitivite, umwihariko, kubyara qPCR.

2. Nta gikorwa cya nuclease exogenous, nta exogenous endo / exonuclease yanduye.

Amakuru ya tekiniki:

1. Superstart qPCR Premix plus-UNG ikoresha enzyme nshya-itangira enzyme ituma ubushyuhe bwihuse butangira muminota 1 ~ 5;binyuze muburyo bwihariye bwa buffer birakwiriye kuri multiplex fluorescent quantitative PCR reaction.

2. Ifite umwihariko wo kunoza cyane ibyiyumvo bya fluorescence ingano ya PCR igarukira, bigatuma umurongo wa amplification ugenda usanzwe, agaciro ka fluorescence gahinduka neza kugaragara mubishusho bito bito, bikwiranye na sensibilité yo hejuru ya fluorescence yuzuye PCR igaragara.

3. Kuri primers ifite ubushyuhe buke bwa annealing cyangwa birenga 200bp ibice, birasabwa uburyo bwintambwe 3.

4. Gukoresha neza imikorere ya dUTP no kumva neza imisemburo ya UNG itandukanye kuri genes zitandukanye, bityo rero niba gukoresha sisitemu ya UNG biganisha ku kugabanuka kwimyumvire, sisitemu yo kubyitwaramo igomba guhinduka kandi ikanozwa.Niba inkunga ya tekiniki isabwa nyamuneka hamagara ikigo cyacu.

5. Koresha uduce twabigenewe hamwe na pipeti mbere na nyuma yo kongererwa imbaraga, ambara uturindantoki mugihe ukora kandi ubisimbuze kenshi;ntugafungure reaction nyuma ya PCR irangiye kugirango ugabanye kwanduza ibicuruzwa bya PCR.