Icyitegererezo cyo Kurekura Reagent
Icyitegererezo cyo Kurekura Reagent ni ya molekuline ya POCT yo gusuzuma.Kuri sisitemu ebyiri zo gukwirakwizwa kwa LAMP itaziguye no kongera PCR mu buryo butaziguye, nta mpamvu yo gukuramo aside nucleic.Lysate ya sample ya sample irashobora kwongerwaho muburyo butaziguye, gene igenewe irashobora gutahurwa neza, igihe cyo kumenya icyitegererezo gishobora kurushaho kugabanywa, gihuye neza nibisabwa na molekile POCT.Irakwiriye kwizuru ryizuru, umuhogo hamwe nubundi bwoko bwikitegererezo.Ingero zitunganijwe zirashobora gukoreshwa muburyo butaziguye bwa fluorescence yuzuye PCR cyangwa LAMP, kandi ibisubizo bimwe nkuburyo busanzwe bwo kuvoma birashobora kugerwaho nta bikorwa bigoye byo gukuramo aside nucleic.
Ububiko
Gutwara no kubika ubushyuhe bwicyumba.
Kugenzura ubuziranenge
Kumenyekanisha imikorere - ingano ya qPCR: Sisitemu ya 800μl Sample Release Reagent yongerewe imbaraga
hamwe na kopi 1000 Novel Pseudovirus, icyitegererezo kimwe cyizuru cya swab, bivamo imirongo isa niyongera kandiΔCt indangagaciro muri ± 0.5 Ct.
Uburyo bw'igeragezwares
1. Fata 800 μl Icyitegererezo cyo Kurekura Reagent hanyuma ukwirakwize igisubizo cya lysis muri 1.5 mL y'icyitegererezo
2. Fata izuru cyangwa umuhogo swab; .
3.Shira swab ako kanya mumashanyarazi.Umutwe wa swab ugomba kuzunguruka no kuvangwa mugisubizo cyo kubika byibuze amasegonda 30 kugirango umenye neza ko icyitegererezo cyatanzwe neza muri tube.
4. Inkubasi ku bushyuhe bwicyumba (20 ~ 25 ℃) kuri 1min, gutegura buffer ya lysis birarangiye.
5. Byombi sisitemu ya 25μl RT-PCR na RT-LAMP byari bihujwe na 10μl ingano yinyongera kugirango igerageze gutahura.
Inyandiko
1. Umubare ntarengwa wa lysate itaziguye ihuye na swab imwe irashobora guhindurwa kuri 400μl, ishobora guhinduka ukurikije ibikenewe kwipimisha.
2. Iyo icyitegererezo kimaze gutunganywa nicyitegererezo cyo kurekura reagent, birasabwa gukora icyiciro gikurikira cyikizamini vuba bishoboka, igihe cyo gutegereza intera ni munsi yisaha 1.
3. pH ya sample lysate ni acide, kandi sisitemu yo gutahura igomba kugira buffer runaka.Irakwiriye kubantu benshi PCR, RT-PCR, na LAMP fluorescence hamwe na pH buffer, ariko ntibikwiriye ko LAMP ibara ryerekana amabara idafite buffer.
