Poly A RNA
Injangwe No: HC4001A
Poly A, polyadenylate, ni uruvange rwa 100 ~ 10000 polyadenylates, ikaba ikozwe na polynucleotide fosifora muri vitro.Muri vivo, poly (a) yongewe kuri 3-terminal ya mRNA na enzyme kugirango iteze imbere mRNA.Mugukoresha acide nucleic acide, kongeramo poly A kuri lysate cyangwa guhuza igisubizo birashobora kuzamura umusaruro wa ADN na RNA.Uburyoya poly A kuzamura umusaruro wa acide nucleic nuburyo bukurikira:
1. Guhuza byuzuye hamwe na adsorption yo hejuru yingingo.Ibintu byinshi bya polypropilene bifite amashanyarazi ahamye hejuru, bizatanga acide nucleic acide.Umwikorezi RNA arashobora kuzuza ibiingaruka za adsorption no kugabanya igihombo cya acide nucleic.
2. Gukora nuclease ya trace: Hariho nuclease zitandukanye mubitegererezo byibinyabuzima kandiibidukikije.Poly A irashobora kudakora nuclease yumurongo mugukuramo cyangwa kubungabunga intambwe kurikuzamura umusaruro no gutuza kwa acide nucleic.
3. Gukoporora: Mu ntambwe yo kweza aside nucleic intambwe ya alcool yifashishije imvura igwa cyangwa igahuza, poly A irashobora kwigana hamwe na aside nucleic igenewe cyangwa igakora uduce twa polymer tokunoza gukira.
Imiterere yo kubika
-20 ~ 8 ℃, kubika byumye, kubika igihe kirekire bigomba gushyirwa kuri -20 ℃
Ibisobanuro
| Umubare CAS | 26763-19-9 |
| Kugaragara | Ifu yera ya lyofilize |
| Isuku | 99% |
| Uburemere bwa molekile | 700-3500 KDa |
Uburyo bwo gukoresha
Fata urugero rukwiye rw'ifu ya lyofilize, ongeramo amazi yatunganijwe DEPC cyangwa umunyu wa guanidinekuyishonga muri 0.1-1ug / uL, hanyuma ukayipakira hanyuma ukayibika kuri -20 ° C.
Porogaramu
1. Gukuramo virusi ya ADN / RNA: kongeramo 1-5ug Carrier RNA kuri lysate birashobora kuzamura umusaruro wa RNA / ADN, bigahindura aside nucleic igamije kandi bikirinda kwangirika kwa acide nucleique isukuye mugihe cyo kubika.
2. Muri micro ADN / RNA ikuramo hakoreshejwe uburyo bwa membrane membrane (<1ug), kongeramo umutwara RNA kuri 1-5ug bifasha kuzamura umusaruro wa acide nucleique.
3. Mu nzoga zahujwe na acide nucleic acide hamwe nintambwe yo kwibandaho, kongeramo 1-2ug itwara RNA ifasha kunoza kugarura igice gito RNA.
4. Muri numero yubushakashatsi bwa PCR ibisubizo, kongeramo 10-100ng itwara RNA mubisubizo byingirakamaro bifasha kunoza sensibilité no kugabanya CTagaciro.
