Intambwe imwe RT-qPCR probe kit
Ibisobanuro
Intambwe imwe qRT-PCR Probe Kit yagenewe byumwihariko kuri qPCR ikoresha mu buryo butaziguye RNA (urugero virusi RNA) nk'icyitegererezo.Ukoresheje primers yihariye (GSP), transcription transcription na qPCR birashobora kurangirira mumiyoboro imwe, bikagabanya cyane uburyo bwo kuvoma no guhura nubwandu.Irashobora kudakorwa kuri 55 ℃ itagize ingaruka kumikorere no kumva neza qRT-PCR.Intambwe imwe qRT-PCR Probe Kit itangwa muri Master mix.5 × Intambwe imwe ivanze ikubiyemo buffer nziza na DNTP / dUTP ivanze, kandi irakwiriye kuri sisitemu yihariye yo gutahura ishingiye kuri fluorescence yanditseho probe (urugero: TaqMan).
Amahame shingiro ya RT-qPCR
Kugaragaza neza
| Ibizamini | Ibisobanuro | Igisubizo |
| (URUPAPURO RWA SDS) Ubuziranenge bwimigabane ya Enzyme (URUPAPURO RWA SDS) | ≥95% | Pass |
| Endonuc Please Igikorwa | Ntibimenyekana | Pass |
| Igikorwa cyo Gusohora | Ntibimenyekana | Pass |
| Igikorwa cya Rnase | Ntibimenyekana | Pass |
| Ibisigisigi bya E.coli ADN | Op 1copies / 60 | Pass |
| Imikorere-Sisitemu | 90% ≤110% | Pass |
Ibigize
| Ibigize | 100rxns | 1.000 | 5.000 rxns |
| Ubusa ddH2O | 2 * 1ml | 20ml | 100ml |
| 5 * intambwe imwe ivanze | 600μl | 6 * 1ml | 30ml |
| Intambwe imwe ya enzyme ivanze | 150μl | 2 * 750μl | 7.5ml |
| 50 * ROX yerekana irangi 1 | 60μl | 600μl | 3 * 1ml |
| 50 * ROX yerekana irangi 2 | 60μl | 600μl | 3 * 1ml |
a.Buffer Intambwe imwe irimo DNTP ivanze na Mg2 +.
b.Enzyme ivanze ahanini irimo revers
transcriptase, Hot Start Taq ADN polymerase (guhindura antibody) na inhibitor ya RNase.
c.Byakoreshejwe mugukosora amakosa yibyaha bya fluorescene hagati yamariba atandukanye.
d.ROX: Ugomba guhitamo kalibrasi ukurikije icyitegererezo cyibikoresho byo gupima.
Porogaramu
Kumenya QPCR
Kohereza no kubika
Kohereza:Amapaki
Uburyo bwo kubika:Ubike kuri -20 ℃.
Ubuzima bwa Shief:Amezi 18
