Virus ADN / Igikoresho cyo gukuramo RNA
Igikoresho (HC1009B) kirashobora gukuramo vuba aside irike ya nucleic acide (ADN / RNA) mubisukari bitandukanye byamazi nkamaraso, serumu, plasma, hamwe na swab yoza amazi, bigatuma itunganywa ryinshi ryikigereranyo.Igikoresho gikoresha superparamagnetic silicon ishingiye kumasaro ya magnetiki.Muri sisitemu idasanzwe ya acide, acide nucleic aho kuba proteyine nizindi mwanda zanduzwa na hydrogène hamwe na electrostatike ihuza.Amasaro ya magnetique afite acide nucleic acide yogejwe kugirango akureho proteine n'umunyu bisigaye.Iyo ukoresheje umunyu muke, acide nucleic irekurwa mumasaro ya magneti, kugirango ugere ku ntego yo gutandukana byihuse no kweza aside nucleique.Ibikorwa byose biroroshe, byihuse, umutekano kandi neza, kandi acide nucleic yabonetse irashobora gukoreshwa muburyo butaziguye mubushakashatsi bwo hasi nko kwandukura ibintu, PCR, qPCR, RT-PCR, RT-qPCR, ibisekuruza bizaza, isesengura rya biochip, n'ibindi
Imiterere yo kubika
Ubike kuri 15 ~ 25 ℃, hanyuma utware ubushyuhe bwicyumba.
Porogaramu
Amaraso, serumu, plasma, swab eluent, tissue homogenate nibindi byinshi.
Inzira y'Ubushakashatsi
1. Icyitegererezo gutunganya
1.1 Kuri virusi ziri mubitegererezo byamazi nkamaraso, serumu, na plasma: 300μL ya ndengakamere ikoreshwa mugukuramo.
2.2 Kuburugero rwa swab: Shyira icyitegererezo cya swab mubitereko byicyitegererezo birimo igisubizo cyo kubungabunga, vortex kuminota 1, hanyuma ufate 300μL ndengakamere kugirango ikurwe.
1.3 Kuri virusi ziri muri tissue homogenates, ibisubizo bya tissueoak, hamwe nicyitegererezo cyibidukikije: Hagarara icyitegererezo kuminota 5 -10, hanyuma ufate 300μL ya supernatant kugirango ikurwe.
2. Gutegura kwa kwitegurareagent
Kuramo reagent zabanje gupakirwa mubikoresho, uhindure hanyuma uvange timesto nyinshi uhagarike amasaro ya magneti.Kunyeganyeza isahani witonze kugirango reagent n'amasaro ya magnetiki bishire munsi y'iriba.Nyamuneka wemeze icyerekezo cy'isahani hanyuma ukureho witonze feri ya aluminium.
Irinde kunyeganyega mugihe ushwanyaguza firime kugirango ushireho amazi.
3. Imikorere ya imashiniigikoresho
3.1 Ongeramo 300μL y'icyitegererezo ku mariba mu nkingi ya 1 cyangwa 7 ya 96 yisahani yimbitse (witondere neza neza neza neza neza).Ingano yinjiza yicyitegererezo irahujwe na 100-400 μL.
3.2 Shyira isahani ya 96-iriba ryimbitse mumashanyarazi acide nucleic.Shyira amaboko ya magnetiki, kandi urebe neza ko apfunduye inkoni za rukuruzi.
3.3 Shiraho gahunda kuburyo bukurikira bwo gukuramo byikora:
3.4 Nyuma yo kuyikuramo, ohereza eluent kuva kumurongo wa 6 cyangwa 12 kuri plaque 96 yimbitse (witondere neza neza aho ikora neza) mumiyoboro isukuye ya Nuclease idafite centrifuge.Niba udahita uyikoresha, nyamuneka ubike ibicuruzwa kuri -20 ℃.
Inyandiko
Kubushakashatsi ukoreshe gusa.Ntabwo ari ugukoresha muburyo bwo gusuzuma.
1. Ibicuruzwa byakuweho ni ADN / RNA.Hagomba kwitabwaho byumwihariko kugirango hirindwe kwangirika kwa RNA na RNase mugihe cyibikorwa.Ibikoresho n'ibikoresho byakoreshejwe bigomba kuba byeguriwe.Imiyoboro yose hamwe ninama za pipette bigomba guhindurwa kandi DNase / RNase-yubusa.Abakoresha bagomba kwambara uturindantoki tutagira ifu na masike.
2. Nyamuneka soma igitabo cyamabwiriza witonze mbere yo gukoresha, kandi ukore ukurikije igitabo gikubiyemo amabwiriza.Gutunganya icyitegererezo bigomba gukorerwa mu ntebe isukuye cyane cyangwa mu biro by’umutekano w’ibinyabuzima.
3. Sisitemu yo gukuramo aside nucleic yikora igomba kwanduzwa na UV muminota 30 mbere na nyuma yo kuyikoresha.
4. Hashobora kubaho ibimenyetso byamasaro ya magneti asigaye muri eluent nyuma yo kuyakuramo, irinde rero kwifuza amasaro ya magneti.Niba amasaro ya magneti yifuzwa, arashobora gukurwaho hamwe na magnetiki.
5. Niba nta mabwiriza yihariye yibice bitandukanye bya reagent, nyamuneka ntuvange, kandi urebe ko ibikoresho byakoreshejwe mugihe cyemewe.
6. Fata neza ibyitegererezo byose hanyuma usubire, uhanagure neza kandi wanduze hejuru yimirimo yose hamwe na 75% Ethanol.
