Taq ADN Irwanya umubiri
Taq ADN Antibody ninzitizi ebyiri zifunga Taq ADN Polymerase antibody ya monoclonal antibody yo gutangira PCR ishyushye.Irashobora guhagarika ibikorwa bya 5 ′ → 3 ′ polymerase na 5 ′ → 3 ′ exonuclease nyuma yo guhambira Taq ADN Polymerase, ishobora guhagarika neza annealing idasanzwe ya primers hamwe na amplification idafite akamaro iterwa na primer dimer ku bushyuhe buke.Byongeye kandi, ibicuruzwa birashobora gukumira neza kwangirika kwa probe.Taq ADN Antibody yamaganwe muntambwe yambere yo gutandukanya ADN ya PCR, aho ibikorwa bya ADN polymerase bigarurwa kugirango bigere ku ngaruka zo gutangira PCR.Irashobora gukoreshwa muburyo bwa PCR isanzwe idakora idasanzwe ya antibody.
Imiterere y'Ububiko
Ibicuruzwa byoherejwe hamwe nudupapuro twa barafu kandi birashobora kubikwa kuri -25 ° C ~ -15 ° C mumyaka 2.
Porogaramu
Ubwinshi bwibicuruzwa ni 5 mg / mL.1 μL antibody irashobora guhagarika ibikorwa bya 20-50 U Taq ADN polymerase.Birasabwa kuvanga antibody na Taq ADN polymerase mubushyuhe bwicyumba kumasaha 1 (incubate kubushyuhe bwicyumba kumasaha 2 mugihe ubunini burenze 200 mL, kandi umukiriya agomba guhindura inzira mugihe akoresheje ubunini bunini), hanyuma akabika kuri -20 ℃ ijoro ryose mbere yo gukoresha.
Icyitonderwa: Igikorwa cyihariye cya Taq ADN Polymerase itandukanye iratandukanye, igipimo cyo guhagarika gikeneye guhindurwa uko bikwiye kugirango intego yo guhagarika iruta 95%.
Ibisobanuro
| Ibyiciro | Monoclonal |
| Andika | Antibody |
| Antigen | Taq ADN Polymerase |
| Ifishi | Amazi |
Inyandiko
Nyamuneka nyamuneka wambare PPE ikenewe, ikote rya laboratoire hamwe na gants, kugirango ubuzima bwawe n'umutekano wawe!
