Ribonuc Please III (RNase III)
Ibisobanuro
Ibicuruzwa ni ribonuclease III (RNase III) byongeye kugaragazwa na E. coli.Iyi exonuclease yihariye ikuramo RNA (dsRNA) ikubye kabiri kandi itanga ibice 12-35bp dsRNA hamwe na 5'-PO4 na 3'-OH, 3 'hejuru
Imiterere yimiti
Igisobanuro
Igikorwa cyibisobanuro: Igice kimwe bivuga ingano ya enzyme isabwa kugirango itesha agaciro 1 μg ya
dsRNA kugeza siRNA muri sisitemu ya 50 μL reaction kuri 37 ° C muminota 20.
Ibisobanuro
| Ibizamini | Ibisobanuro |
| Exonuclease Igikorwa | Isohora ＜ 0.1% ya radioactivite yose |
| Igikorwa kidasanzwe cya Dnase | Ntibishobora kumenyekana |
| Isuzuma rya poroteyine (SDS-PAGE) | ≥ 95% |
| Igikorwa cya RNase (Kwagura Byagutse) | ＞ 90% ya substrate RNA ikomeza kuba ntamakemwa |
Gutwara no kubika
Ubwikorezi:Urubura rwumye
Ububiko:Ubike kuri -25 ~ -15 ° C.
Basabwe kongera kugerageza Ubuzima:Imyaka 2
ibicuruzwa bifitanye isano
Andika ubutumwa bwawe hano hanyuma utwohereze
