DNase I (Rnase Ubuntu) (2u / ul)
Injangwe No: HC4007B
DNase I ni endonuc Please ishobora gusya ADN imwe cyangwa ebyiri.Irashobora hydrolyze guhuza fosifori kugirango itange mono-na oligodeoxynucleotide irimo 5'-fosifate nitsinda rya 3'-OH.Uburyo bwiza bwo gukora pH urwego rwa DNase I ni 7-8.Igikorwa cya DNase I giterwa na Ca.2+kandi irashobora gukoreshwa na ion zingana nicyuma nka CO2, Mn2+, Zn2+, nibindi imbere ya Mg2+, DNase Nshobora gutandukanya urubuga urwo arirwo rwose rwa ADN ebyiri;Mugihe imbere ya Mn2+, DNase Nshobora gukuramo ADN ikubye kabiri kurubuga rumwe, nkora imitwe idahwitse cyangwa impera zifatika hamwe na nucleotide 1-2 zisohoka.Irashobora gukoreshwa mugutunganya ingero zitandukanye za RNA.
Ibigize
| Izina | 1KU | 5KU |
| Recombinant DNaseI (RNase-yubusa) | 500 μL | 5 × 500 μL |
| DNase I reaction Buffer (10 ×) | 1 mL | 5 × 1mL |
Imiterere yo kubika
Ibicuruzwa bigomba kubikwa kuri -25 ~ -15 ℃ kumyaka 2.Nyamuneka wirinde guhagarika inshuro nyinshi.
Amabwiriza
Byakoreshejwe mugukuraho ADN kuburugero rwa RNA kugirango ikoreshwe gusa.
1. Nyamuneka koresha imiyoboro ya centrifuge ya RNase idafite inama na pipette kugirango utegure sisitemu ikurikira:
| Ibigize | Umubumbe (μL) |
| DNase I reaction Buffer (10 ×) | 1 |
| Recombinant DNasel (RNase-yubusa) | 1 |
| RNA | X |
| Ubusa ddH2O | Kugera kuri 10 |
2. Imiterere yimyitwarire nuburyo bukurikira: 37 ℃, nyuma yiminota 15-30, ongeramo intumbero yanyuma ya 2.5 mM EDTA yumuti hanyuma uvange neza, hanyuma 65 ℃ kuminota 10.Inyandikorugero yatunganijwe irashobora gukoreshwa mubushakashatsi bwa RT-PCR cyangwa RT-qPCR, nibindi.
Inyandiko
1. DNase l yunvikana no gutandukana kumubiri;Mugihe cyo kuvanga, hindura buhoro buhoro umuyoboro wikizamini nakunyeganyeza neza, ntugahungabanye cyane.
2. Enzyme igomba kubikwa mu gasanduku ka barafu cyangwa ku bwogero bwa barafu iyo ikoreshejwe, kandi igomba kubikwa kuri -20 ℃ ako kanya nyuma yo kuyikoresha.
3. Iki gicuruzwa nikoreshwa mubushakashatsi gusa.
4. Nyamuneka kora ikoti ya laboratoire hamwe na gants imwe ikoreshwa, kubwumutekano wawe.
