Fosifata ya alkaline (ALP)
Ibisobanuro
Alkaline Phosphatase ikomoka kumurongo wa recombinant E. coli itwara gene TAB5.Enzyme itera dephosifora ya 5´ na 3´ ya ADN na fosifomone ya RNA.Nanone, hydrolyses ribose, kimwe na deoxyribonucleoside triphosphates (NTPs na dNTPs).TAB5 Fosifata ya alkaline ikora kuri 5´ isohoka, 5´ isubirwamo kandi itagaragara.Fosifata irashobora gukoreshwa mubikorwa byinshi bya biyolojiya ya biologiya, nka clon cyangwa probe end labels kugirango ikureho fosifora ya ADN cyangwa RNA.Mu bushakashatsi bwakoronijwe, dephosifora irinda ADN umurongo wa plasmid umurongo wo kwikuramo.Irashobora kandi gutesha agaciro dNTPs zidafite uruhare muri PCR kugirango zitegure icyitegererezo cya ADN ikurikirana.Enzyme irakorwa rwose kandi kuburyo budasubirwaho no gushyushya 70 ° C muminota 5, bityo bigatuma ikurwaho rya fosifata mbere yo kuburana cyangwa kurangiza kuranga bitari ngombwa
Ikoreshwa
1.Alkaline fosifata ihujwe na poroteyine (antibodies, streptavidin nibindi,) irashobora kumenya neza molekile zigenewe, kandi irashobora gukoreshwa muri ELISA, WB no kumenya amateka ya histochemiki;
2.Alkaline fosifata irashobora gukoreshwa mugutandukanya 5 '-imiterere ya ADN cyangwa RNA kugirango wirinde kwihuza;
3.A ADN ya dephosifora yavuzwe haruguru cyangwa RNA irashobora gushyirwaho ikimenyetso cya fosifeti ya radio (binyuze kuri T4 poly-nucleotide kinase)
Imiterere yimiti
Ibisobanuro
| Ibizamini | Ibisobanuro |
| Igikorwa cya Enzyme | 5U / μL |
| Igikorwa cya Endonuc Please | Ntibimenyekana |
| Igikorwa cya Exonuuc Please | Ntibimenyekana |
| Igikorwa cyo Kwitonda | Ntibimenyekana |
| Igikorwa cya RNase | Ntibimenyekana |
| E.coli ADN | ≤1copy / 5U |
| Endotoxin | LAL-Ikizamini, ≤10EU / mg |
| Isuku | ≥95% |
Gutwara no kubika
Ubwikorezi:AmbIent
Ububiko:Ubike kuri 2-8 ° C.
Basabwe kongera gukora ikizaminiUbuzima:Imyaka 2
