Hotstart Taq ADN Polymerase (5u / ul)
Taq ADN Polymerase ni intangiriro ishyushye ya polymerase ya ADN hamwe no guhagarika kabiri na antibodi ebyiri.Ibicuruzwa ntibibuza gusa ibikorwa bya 5 ′ → 3 ′ polymerase ya Taq DNA polymerase, ahubwo binabuza ibikorwa 5 ′ → 3′ibisobanuro.Gushyushya amasegonda 30 kubushyuhe bwa pre-denaturation birashobora gukora rwose antibody no kurekura ibikorwa bya ADN polymerase nibikorwa bya exonuclease.Guhagarika inshuro ebyiri biranga ntibishobora gusa gukumira neza amplification idafite akamaro iterwa no kudahuza cyangwa primer dimer, ariko kandi irashobora kubuza neza kugabanuka kwikimenyetso cya fluorescence iterwa no kwangirika kwa probe, kugirango itume in vitro itahura neza mugihe cyo gutwara cyangwa gukoresha mucyumba ubushyuhe.
Ibigize
| Ibigize | HC1012B (250U) | HC1012B (1000U) | HC1012B (10000U) | HC1012B (25000U) |
| Taq ADN Polymerase(5 U / μL) | 50 μL | 200 μL | 2 mL | 5 mL |
Imiterere y'Ububiko
Ibicuruzwa byoherejwe hamwe na barafu yumye kandi birashobora kubikwa kuri -25 ° C ~ -15 ° C mumyaka 2.
Ibisobanuro
| Polymerase | Taq ADN Polymerase |
| Isuku | ≥ 95% (SDS-PAGE) |
| Intangiriro ishyushye | Byubatswe muri Hot Itangira |
| Umuvuduko wo Kwitabira | Bisanzwe |
| Exonuclease Igikorwa | 5 ′ → 3 ′ |
Amabwiriza
Gushiraho
| Ibigize | Umubumbe (μL) | Kwibanda kwanyuma |
| 2 Buffera | 25 | 1 × |
| Primer / Probe ivanzeb | × | 0.1 μ mol / L-0.5 μ mol / L. |
| Hotstart Taq Polymerase (5U / μL) | 1.2 | 0.12 U / μL |
| Icyitegererezo cya ADNc | × | 0.1-100 ng |
| ddH2O | Kugera kuri 50 | - |
Inyandiko:
1) Ukurikije uburyo bwihariye bwo kugerageza, birakenewe gutegura buffer reaction.
2) Ingano ya ADN hamwe nubushakashatsi bwa probe cyangwa primers birasabwa kwibanda.Ibyifuzo byiza birashobora guhinduka ukurikije ibihe byubushakashatsi.
Umukino wo gusiganwa ku magare
| Intambwe | Ubushyuhe(° C.) | Igihe | Amagare |
| Mbere yo gutandukana | 95 ℃ | 5min | 1 |
| Gutandukana | 95 ℃ | 15 amasegonda | 45 |
| Kwiyongera / Kwagura | 60 ℃a | 30 amasegondab |
Inyandiko:
1) Ubushyuhe bwa reaction bwahinduwe ukurikije agaciro ka Tm ya primers yagenewe.
2) Ibikoresho bitandukanye bya qPCR bikenera igihe cyo kubona ibimenyetso bya fluorescence bitandukanye, nyamuneka shiraho ukurikije igihe gito.
Inyandiko
Nyamuneka nyamuneka wambare PPE ikenewe, ikote rya laboratoire hamwe na gants, kugirango ubuzima bwawe n'umutekano wawe!
