Hexokinase (HK)
Ibisobanuro
Koresha Hexokinase kugirango umenye D-glucose, D-fructose, na D-sorbitol mubiribwa cyangwa ubushakashatsi bwibinyabuzima.Enzyme ikoreshwa kandi mugusuzuma izindi sakaride zihinduka glucose cyangwa fructose, bityo ikaba ingirakamaro mugusuzuma glycoside nyinshi.
Niba Hexokinase ikoreshwa ifatanije na glucose-6-fosifate dehydrogenase (G6P-DH) * (ivuga ko glucose6-fosifate yakozwe na Hexokinase), ingero ntizigomba kuba zifite fosifate nyinshi kuko G6P-DH ihagarikwa na fosifate.
Imiterere yimiti
Ihame ry'imyitwarire
D-Hexose + ATP -Mg2 +→ D-Hexose-6-fosifate + ADP
Ibisobanuro
| Ibizamini | Ibisobanuro |
| Ibisobanuro | Umweru kugeza muto ifu ya amorphous, lyofilize |
| Igikorwa | ≥30U / mg |
| Isuku (SDS-PAGE) | ≥90% |
| Gukemura (10mg ifu / ml) | Biragaragara |
| Protease | ≤0.01% |
| ATPase | ≤0.03% |
| Foshoglucose isomerase | ≤0.001% |
| Kurema fosifokine | ≤0.001% |
| Glucose-6-Fosifate dehydrogenase | ≤0.01% |
| NADH / NADPH okiside | ≤0.01% |
Gutwara no kubika
Ubwikorezi: Ambient
Ububiko:Ubike kuri -20 ° C (Igihe kirekire), 2-8 ° C (igihe gito)
Basabwe kongera gukora ikizaminiUbuzima:Umwaka 1
ibicuruzwa bifitanye isano
Andika ubutumwa bwawe hano hanyuma utwohereze
