Glycohemoglobin A1c (HbA1c) Kit Ikizamini
Ibyiza
Ukuri neza
Ubushobozi bukomeye bwo kurwanya kwivanga
Stability Guhagarara neza
Imiterere yimiti
Porogaramu
Mu isuzuma rya vitro kugirango hamenyekane ingano yo kwibanda kwa HbA1c mumaraso yabantu yose kuri sisitemu yo gufotora.HbA1c nigicuruzwa cya hemoglobine (Hb) itanga gahoro gahoro kandi idahoraho idafite glycation munsi ya glucose yamaraso.Glucose ihindura hemoglobine cyane mubisigisigi byayo bya n-terminal kugirango ikore gemoglobine.Mubihe bisanzwe byimiterere yumubiri, umusaruro wibintu bya glycosylation bitari enzymatique bigereranywa neza nubushakashatsi bwibintu.Kubera ko intungamubiri za hemoglobine zikomeza kuba zihamye, urugero rwa glycosylation rushingiye ahanini ku kwibanda kwa glucose kandi bifitanye isano n'uburebure bwa hemoglobine na glucose.Kubwibyo, HbA1c nikimenyetso cyiza kumpuzandengo ya glucose yamaraso yabarwayi mumezi 2 ~ 3 ashize.
Ihame
Mubikorwa bya protease, n-terminal ya β urunigi muri HbA1c iracibwa hanyuma dipeptide ya glycosylated irekurwa.Mubisubizo byambere, kwibanda kwa Hb birashobora kuboneka mugupima kwinjiza 480 nm.Mu gisubizo cya kabiri, fructosyl peptide oxydease (FPOX) ikora kuri dipeptide ya glycosilated kugirango itange hydrogène peroxide ishobora gukora hamwe na chromogeneque kugirango itange kwinjiza kuri 660nm imbere ya peroxidase, hanyuma ubunini bwa HbA1c burashobora kuboneka mugupima kwinjiza kwa 660nm.Ukurikije ubunini bwa HbA1c hamwe nubushakashatsi bwa Hb, ijanisha rya HbA1c (HbA1c%) rirashobora kubarwa.
Birashoboka
Hitachi 7180/7170/7060/7600 isesengura ryibinyabuzima ryibinyabuzima 、 Abbot 16000 、 OLYMPUS AU640 isesengura ryibinyabuzima ryibinyabuzima
Reagents
| Ibigize | Kwibanda |
| Reagent 1 (R1) | |
| Buffer nziza | 100mmol / L. |
| PRK | 500KU / L. |
| DA-67 | 10mmol / L. |
| Reagents 2 （R2） | |
| Buffer nziza | 100mmol / L. |
| Fructosyl peptide oxyde | 50 KU / L. |
| Reagent 3 (R3) | |
| Buffer nziza | 100mmol / L. |
Gutwara no kubika
Ubwikorezi:Ibidukikije
Kubika no Guhagarara:
Kugeza igihe cyo kurangiriraho cyerekanwe kuri label, iyo kibitswe kidafunguwe kuri 2-8 ℃ kandi kirinzwe kumucyo.Bimaze gukingurwa, reagent ihagaze muminsi 28 iyo ikonjeshejwe kuri analyseur cyangwa firigo.
Kwanduza reagent bigomba kwirindwa.Ntugahagarike reagent.
Iyo bimaze gushonga, kalibatori ihagaze muminsi 15 kuri 2-8 ℃, igenzura rihagaze muminsi 7 kuri 2-8 ℃ ， ntukonje.
Ubuzima bwa Shelf:Umwaka 1
