Peroxidase (Inkomoko ya Horseradish) Synonym: Hydrogen peroxide oxidoreductase;HRP

Horseradish peroxidase (HRP) yitaruye mu mizi ya horseradish (Amoracia rusticana) kandi iri mu itsinda rya ferroprotoporphyrin ya peroxidase.HRP byoroshye guhuza hydrogen peroxide (H2O2).Ibisubizo bivamo [HRP-H2O2] birashobora guhumeka ubwoko butandukanye bwa hydrogène:
Abaterankunga + H2O2 → Umuterankunga Oxidized + 2 H2O
HRP izahindura ibice bitandukanye (reba Imbonerahamwe 1):
Chromogenic
• Chemiluminescent (nka luminol cyangwa isoluminol)
• Fluorogenic (nka tyramine, aside homovanillic, cyangwa acide acide 4-hydroxyphenyl)
HRP ni urunigi rumwe polypeptide irimo ibiraro bine bya disulfide.HRP ni glycoproteine ​​irimo 18% bya karubone.Carbohydrate igizwe na galactose, arabinose, xylose, fucose, mannose, mannosamine, na galactosamine, bitewe na isozyme yihariye.

HRP ni ikirango gikoreshwa cyane kuri immunoglobuline mubikorwa byinshi bitandukanye bya immunochemie, harimo immunoblotting, immunohistochemie, na ELISA.HRP irashobora guhuzwa na antibodies muburyo butandukanye, harimo glutaraldehyde, okiside yigihe, binyuze mumigozi ya disulfide, kandi ikoresheje amino na thiol iyobora.HRP ni ikirango cyifuzwa cyane kuri antibodies, kubera ko ari ntoya kandi itajegajega muri label eshatu zizwi cyane za enzyme (peroxidase, β-galactosidase, fosifata ya alkaline) kandi glycosylation yayo iganisha kumurongo wo hasi udasanzwe.Isubiramo rya glutaraldehyde nuburyo bwa conjugation burigihe byashyizwe ahagaragara.

Peroxidase nayo ikoreshwa muguhitamo glucose4 na peroxide mugisubizo.Ibisohokayandikiro byinshi, 6-24 theeses, 25-29 na dissertations 30-46 bavuze ko gukoresha P8375 muri protocole yabo yubushakashatsi.